Complete workflow solution for large-scale 3D fluorescence microscopy A journey into 3D light sheet microscopy: Register for our webinar series today. Simplify T cell activation and expansion with T Cell TransAct™ for Research & Clinical use. For a chance to win a Happy Cell plush toy: Comple...
Expansion microscopyLight sheet fluorescence microscopySerial gel block imagingCal-lightDeepLabStreamFunctional connectomedoi:10.1016/j.pbiomolbio.2021.09.004Martin K. SchwarzInstitute Experimental Epileptology and Cognition Research (EECR), University of Bonn Medical School, Sigmund-Freud-Str. 25, 53127, ...
Fig. 1: Schematic diagram of a remote focusing system implemented in light-sheet microscopy and its performance. a Three different modalities to acquire volumetric imaging of the sample along the focus direction. Either the sample or objective lens (obj) can be moved for axial re-focusing. Alter...
SecMRA反卷积后的宽场图像可以达到类似共聚焦显微镜的层切效果,可以在提供高质量图像的同时进一步降低活细胞成像的光毒性。作者在宽场、Lightsheet、Confocal、SoRa、SIM、STED和Expansion显微镜等多种成像模态中验证了该方法的有效性。 作者展示了利用MRA反卷积...
3D molecular phenotyping of cleared human brain tissues with light-sheet fluorescence microscopy ArticleOpen access12 May 2022 Introduction Since the early attempts of organ-scale three-dimensional (3D) imaging with light-sheet fluorescence microscopy (LSFM)1, tissue clearing has become the gold standard...
light sheet microscopy tiling light sheet microscopy tiling light sheet selective plane illumination microscopy tissue clearing tissue expansion cleared tissue imaging Introduction High-resolution 3D fluorescence imaging of biological tissues bridges the study of gene expression, cell morphology, and cell distri...
Light sheet microscopy, Whole brain imaging, Quantitative imaging, Hydra, Calcium imaging, Tissue clearing, Expansion microscopyBackground Advances in tissue clearing and molecular labeling methods are enabling unprecedented optical access to large intact biological systems. These developments fuel the need ...
Lightsheet localization microscopy enables fast, large-scale, and three-dimensional super-resolution imaging. Commun. Biol. 2, 177 (2019). Article PubMed PubMed Central Google Scholar Planchon, T. A. et al. Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane ...
ClearScope is a revolutionary light sheet theta microscope system designed to quickly and easily image cleared specimens of nearly any size at subcellular resolution.
The scattering of a non-paraxial Bessel light-sheet by a sphere of arbitrary size is studied in framework of generalized Lorenz-–Mie theory (GLMT). The electrical fields of the Bessel light-sheet are expanded using the vector angular spectrum decomposition method (VASDM), and the beam shape...