Galileo Galilei invented the first microscope "occhiolino," by combining a concave and a convex lens in 1600s. Robert Hooke and Anton van Leeuwenhoek later modified it to look at living things. Since then, light microscopy has gained immense popularity and has been pushing the limits of optical...
Resolution doubling in light-sheet microscopy via oblique plane structured illumination The longstanding goal of combining the optical sectioning of light-sheet illumination and the resolving power of multidirectional structured illumination microscopy is realized using an oblique plane microscope for improved...
Super-resolution microscopy is an optical imaging technology that overcomes the diffraction limit of light and allows the visualization of subcellular structures and dynamics in greater detail than can be achieved with conventional optical microscopy. A resolution of 30 nm is possible usingSTED(stimulated...
Light-field microscopy (LFM) is a functional, plenoptic imaging technique which offers ultrahigh spatial and temporal resolution for 3D tissue samples. Lotus is an ambitious new imaging project at LeafLabs, the goal is to use light-field microscopy to record spikes from every neuron in the zebrafi...
Light-sheet fluorescence microscopy (LSFM) enables high-speed, high-resolution, and gentle imaging of live specimens over extended periods. Here we describe a technique that improves the spatiotemporal resolution and collection efficiency of LSFM without modifying the underlying microscope. By imaging sam...
A microscope may offer high magnification, but if the lenses are of poor quality the resulting poor resolution will degrade the image quality. Below is Abbe's equation in order to calculate approximate resolving power: resolving power = wavelength of light used/2 (numerical aperture of objective)...
More image detail will be resolved in a microscope system in which all of the optical components are correctly aligned, have a relatively high NA value and are working harmoniously with each other. Resolution is also related to the wavelength of light which is used to image a specimen;...
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Mitochondria in a live adherent human cancer cell (HeLa). Cells were stained with the fluorescent probe PK Mito Orange and recorded on a stimulated emission depletion (STED) microscope (Abberior Instruments Expert Line) using 561 nm excitation and 775 nm depletion light and an UPlanSApo 100×/...
Gerchberg and Saxton (GS) [2] took advantage of this possibility by measuring not only the intensity of the wave in the image plane (observation plane) but also the intensity of the wave in the Fourier plane (initial plane) in the electron microscope. The result provided an answer for ...