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6.2.1.1 Light microscopy Light microscopy is based on light production from a visible light source that passes through a condenser lens. Light passes through the thin sample and, through an objective lens, is focused on forming an enlarged image. The resolution of this technique is 1000 times ...
Here, using high spatiotemporal resolution light-sheet and fluorescence microscopy, the authors investigate the role of cytoskeletal components on the intracellular transport of Rab11A and influenza virus (IAV) vRNP), and show a preference for Rab11A movement along microtubules that is not essential...
1.Hell, S. W. & Wichmann, J. Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy.Opt Lett19, 780–782 (1994). 2.Huang, X.et al.Fast, long-term, super-...
Rheinberg optical staining, DPC, TIE quantitative phase microscopy, and FPM. In addition, the use of partially coherent LED illumination endows MQP-SCLM with higher phase imaging resolution (up to incoherent diffraction limit) and speckle-free image quality, which is extremely valuable for microscopic...
The transport of intensity equation (TIE) is a well-established phase retrieval technique that enables incoherent diffraction limit-resolution imaging and is compatible with widely available brightfield microscopy hardware. However, existing TIE methods encounter difficulties in decoupling the independent contr...
Push the boundaries of research with reliable, precise and versatile light sheet microscopy solutions from Leica Microsystems to study developing embryos, spheroids, organoids or other delicate samples. Intestinal organoids label with FUCCI reporter to follow cell cycle dynamics. Courtesy of Franziska Moo...
Non-invasive deep-tissue three-dimensional optical imaging of live mammals with high spatiotemporal resolution is challenging owing to light scattering. We developed near-infrared II (1,000–1,700 nm) light-sheet microscopy with excitation and emission of up to approximately 1,320 nm and 1,...
Laser manufacturing of spatial resolution approaching quantum limit 清华大学孙洪波和方红华等 ① 阅读本文详细解读▼点击下划线文字 超快激光加工精度接近量子极限 ② 阅读论文原文▼点击下划线网址 https://doi.org/10.1038/s41377-023-01354-5 「相关论文信息」Wang, XJ., F...
(HSQ) metasurface consists of an azimuthally gradient width.c.iSEM image of metasurfaces before 2D emitter integration.c.iiFabrication process of metasurface-integrated 2D emitter. LM: layered materials. PDMS: polydimethylsiloxane.c.iiiDark field optical microscopy images of the metasurface-integrated ...