Figure 1.A light microscope; 1, light source; 2, condenser; 3, stage; 4, objective lenses; 5, ocular lenses; 6, screw used to focus. The magnification is directly linked to resolution or resolving power defined as the smallest distance between two points at which they can be separately ...
The present invention relates to an apparatus for the optical analysis of a sample, also referred to as microscope, which is configured for an optical analysis process having high resolution for the detection of fluorescent molecules. The apparatus and the process applied when using the apparatus ...
In general, light microscopy is limited by both the range of elements for which stains are available and by the resolution of the light microscope, which confines observations at best to the level of individual cells. For unambiguous detection of elements and their localization at the cellular ...
Light-sheet single-molecule 3D super-resolution microscopes can’t image close to a coverslip or may require complex apparatus. Here the authors overcome such limitations using a tilted light sheet strategy with long axial range point spread functions on a standard inverted microscope. Anna-Karin Gus...
Light-sheet microscopy uses a thin plane of light to illuminate a sample orthogonally to the detection objective such that the axial resolution of the microscope is determined wholly or partly by the thickness of the light sheet. It allows fast volumetric imaging with reduced sample irradiation comp...
(Λ = 0.28 µm,h> 50 nm). Here, the 3D micro-device proved to be capable of enhancing lateral resolution beyond the limits achievable with conventional lenses or microscope objectives when used in air. Simultaneously, it maintained the high axial resolution characteristic of Mirau-type ...
2) superresolution microscope 超高分辨光学显微镜3) high resolution electron microscope 高分辨率电子显微镜 例句>> 4) surper resolution optical microscope 超分辨光学显微镜5) high-resolution microscopy 高分辨显微镜6) high-resolution TEM 高分辨率透射电子显微镜...
microscope. For example, a spider stop is inserted into the aperture focal plane of the condenser to create a hollow cone of illumination with larger NA compared to that of the objective lens, extracting high-resolution detailed information of the specimen. Computational light microscopy brought a ...
aMicroscope objectives (MOs) must create a high-quality image, corresponding to the diffraction limit of resolution: , where λ is the wavelength of the light, and A is the numerical aperture of the MO. Because of the existing theory of image formation in a microscope and the availability of...
Evanescent waves were then used in the TIRF microscope as the illumination light. Although the TIRF microscope does not utilize the full effectiveness of the evanescent wave for super-resolution, the chief advantages of this development are a thin optical sectioning and a lower background noise, bo...