From existing therapies to those currently in clinical trials, lentiviral vector adherent cell culture continues to be dependable way to develop and study innovative therapeutic interventions in healthcare. Lentiviral vector adherent cultures offer specific properties that make them ...
lentiviral vectorgene therapyprimary immunodeficiency diseasesleukemiahemoglobinopathiesneurodegenerative diseasesLentiviral vectors(LVs), derived from human immunodeficiency virus, are powerful tools for modifying the genes of eukaryotic cells such as hematopoietic stem cells and neural cells. With the extensive ...
with predominant expression in immune cells and minimal proximal enhancers to improve vector safety. This lentiviral vector efficiently transduces major dendritic cell subsets in vivo. With a mycobacterial immunogen, we observed distinct functional signatures and memory phenotype in lentiviral vector- or Ade...
Lentiviral vector-based insertional mutagenesis identifies genes associated with liver cancer. Ranzani Marco,Cesana Daniela,Bartholomae Cynthia C,Sanvito Francesca,Pala Mauro,Benedicenti Fabrizio,Gallina Pierangela,Sergi Lucia Sergi,Merella Stefania,Bulfone Alessandro,Doglioni Claudio,von Kalle Christof,...
Second, they do not transfer any viral gene, thus alleviating the risk of vector-mediated toxicity and immune response against the transduced cells. Both of these properties are crucial for achieving sustained expression of the transgene. The retroviral vectors used in clinical trials have been ...
Keywords Cancer therapy Clinical translation Gene delivery Lentiviral vector Pseudotype Background Delivering curative genetic material into deleterious cells to combat disease, i.e., gene therapy, has shown remarkable therapeutic benefit and safety record in clinical trials, and is also rapidly emerging ...
DISCUSSION According to the Gene Therapy Clinical Trials Worldwide database provided by the Journal of Gene Medicine, there is a growing interest in LVs, and they are considered as a promising vector type for gene therapy.1,2 However, LV production methods need to be upgraded for efficient ...
Lentiviral vector (LV)-based hematopoietic stem and progenitor cell (HSPC) gene therapy is becoming a promising alternative to allogeneic stem cell transplantation for curing genetic diseases. Clinical trials are currently underway to treat sickle cell disease using LVs expressing designed anti-sickling ...
Regulatory considerations for novel gene therapy products: a review of the process leading to the first clinical lentiviral vector. process and major safety concerns, toward providing useful insight to those presently embarking on the development of clinical application of lentiviral vectors... P Manilla...
The invention relates to the regulated expression of a chimeric antigen receptor (CAR) within a lentiviral vector. The CAR comprises a hook-binding domain that interacts with a hook, preferably encoded by the same lentiviral vector, which prevents proper processing and release of the CAR to the ...