vectorcancergene therapyRetroviruses have been widely used as gene transfer vectors, and in fact represent the vector system used in the majority of clinical gene therapy trials for cancer to date. In an ex vivo setting, conventional replication-defective oncoretrovirus vectors can reliably and ...
nativeepitopesisrequired.Theproductionofnovelpseudotypedvectorsisparticularlyusefulfortheexpansionoftissuetypesthatcanpotentiallybetargetedbyvirus-basedgenetherapyvectors(29).Retroviralvectorspermittheefficienttransductionofabroadarrayofcelltypes.Originally,retroviralvectorsbasedonmurineoncoret-roviruseswereusedinanumber...
Retroviral vector production under serum deprivation: the role of lipids. Biotechnol Bioeng 2009; 104: 1171–1181. 20 Lennaertz A, Knowles S, Drugmand J-C, Castillo J. Viral vector production in the integrity® iCELLis® single-use fixed-bed bioreactor, from bench-scale to industrial scale...
Conclusions: Lentiviral gene transfer represents a feasible pathway to glioma modeling in higher order species. The present model is the first lentiviral vector induced pig model of high-grade spinal cord glioma and may potentially be used in preclinical therapeutic development programs....
lentivirus vector retroviral vector complement resistance human sera inactivation VSV G pseudotyping Introduction Two important recent advances in retroviral vector technology are (i) the development of stable human packaging cell lines (PCLs) for production of Moloney murine leukemia virus (MLV) vectors...
Challita PM, Kohn DB: Lack of expression from a retroviral vector after transduction of murine hematopoietic stem cells is associated with methylation in vivo. Proc Natl Acad Sci USA. 1994, 91: 2567-71. 10.1073/pnas.91.7.2567. Article PubMed Central CAS PubMed Google Scholar Jähner D, ...
A comparison of expression levels revealed that integrated lentivectors express more transgene protein per vector copy than NILVs and AAV vectors, but both episomal vectors display similar levels of transgene expression per vector copy.In conclusion, NILVs have the potential to be used as tools for...
Recently it has been reported that vector efficacy reduces in correlation with an increase in the size of vector RNA and that lentiviral vector integration is impeded when delivering large transgenes19,20. This suggests that vector packaging limits could be circumvented by reducing the amount of ...
In the present disclosure, transgenes may comprise viral genes (e.g., retroviral genes). Transgenes of the present disclosure encode packagable vector RNA. As used herein, “packagable vector RNA” refers to RNA encoding any genetic element, such as a virus, virion, capsid, etc., that is...
at 96 hrs (β2M vector at MOI=3 and UBC vector MOI=3 and 10) and at 8 days (β2M vector at MOI=30 and UBC vector at every MOI tested). At