vectorcancergene therapyRetroviruses have been widely used as gene transfer vectors, and in fact represent the vector system used in the majority of clinical gene therapy trials for cancer to date. In an ex vivo setting, conventional replication-defective oncoretrovirus vectors can reliably and ...
Lentiviral vectors were produced by transient cotransfection of 293T cells with the packaging vector pCMV-ΔD8.9,20 the VSV-G envelope encoding vector pMD.G,19 and the corresponding backbone vector pHR-CMV-EGFP,25 pHR-CD80, pHR-GM-CSF, or PHR-GM/CD (Figure 1). Supernatant viral titer an...
26,27. For this reason, the SIN vector genome plasmid pRRLSIN.cPPT.PGK-GFP.WPRE (Fig.1d) was used as vector backbone. Following the rational used with the other LV components, an antibiotic resistance marker was introduced into this vector...
48 This process, known as pseudotyping, allows retroviral vectors to transduce a broader range of cells and tissues. Engineering of lentiviral vectors with the VSV-G GP exemplifies the ability of a heterologous GP to extend the tropism of a vector.2 However, coexpression of a given GP with ...
The present invention provides a retroviral or lentiviral vector having a viral envelope which comprises a mitogenic T-cell activating transmembrane protein which comprises: (i) a m
Cell Cycle Control and Dysregulation Protocols Volume 285 || Retroviral and Lentiviral Vector Titration by the Analysis of the Activity of Viral Reverse Tr... A Giordano,G Romano 被引量: 0发表: 2004年 Improved methods of retroviral vector transduction and production for gene therapy. To ...
vector efficacy reduces in correlation with an increase in the size of vector RNA and that lentiviral vector integration is impeded when delivering large transgenes19,20. This suggests that vector packaging limits could be circumvented by reducing the amount of RNA to be packaged into a vector ...
The present invention provides a retroviral or lentiviral vector having a viral envelope which comprises: (i) a mitogenic T-cell activating transmembrane protein which comprises a m
The overlap of EFS-RP19 insertions with the Retroviral Tagged Cancer Gene Database (RTCGD)32 or the All Onco cancer gene list33 was not different from a randomized control dataset (Figure 7B). We did not observe a significant difference in clonal diversity between the vector groups. However,...
The lentiviral vector may be present in the form of an RNA or DNA molecule, depending on the stage of production or development of said retroviral vectors. The lentiviral vector can be in the form of a recombinant DNA molecule, such as a plasmid. The lentiviral vector can be in the form...