A comparison of expression levels revealed that integrated lentivectors express more transgene protein per vector copy than NILVs and AAV vectors, but both episomal vectors display similar levels of transgene expression per vector copy.In conclusion, NILVs have the potential to be used as tools for...
Naldini L, Blomer U, Gage FH, Trono D, Verma IM. Efficient transfer, integration, and sustained long-term expression of the transgene in adult rat brains injected with a lentiviral vector. Proc Natl Acad Sci USA 93:11382–11388;1996. Pappalardo L, Kerwood DJ, Pelczer I, Borer PN. Thre...
Comparative analysis of the transduction efficiency of five adeno associated virus serotypes and VSV-G pseudotype lentiviral vector in lung cancer cells. Virol J. 2013;10:86. : 10.1186/1743-422x-10-86 . CentralComparative analysis of the transduction efficiency of five adeno associated ...
Stable gene transfer to the nervous system using a non-primate lentiviral vector. We have constructed a non-primate lentiviral vector system based on the equine infectious anaemia virus (EIAV). This system is able to transduce both divid... Mitrophanous,K A,Yoon,... - 《Gene Therapy》 被...
A lentiviral vector strategy for efficient gene transfer through retrograde axonal transport provides a powerful approach for studying the neural circuit mechanisms that mediate higher level functions of the central nervous system. Pseudotyping of human immunodeficiency virus type-1 with different types of ...
Therapeutic genome editing requires effective and targeted delivery methods. The delivery of Cas9 mRNA using adeno-associated viruses has led to potent in vivo therapeutic efficacy, but can cause sustained Cas9 expression, anti-Cas9 immune responses and
In addition, adenoviral vector production has been scaled-up to iCELLis 500.15 Here, for the first time, LV production was optimized for scale- up using iCELLis Nano in perfusion setting in adherent 293T cells. Process was designed to be adaptable to iCELLis 500. Both CaPho precipitation and ...
described a lentiviral vector able to undergo gene targeting by HR after I-Sce1 activity.27 An I-Sce1 expression cassette and a homologous repair template were cloned into independent NILVs. Gene conversion at a chromosomal enhanced green fluorescent protein target site was observed in ∼1% of...
administration of an adeno-associated virus (AAV) vector delivering a functional FIX or FVIII transgene to the liver, in adults affected by severe hemophilia B or A, respectively4,5,6. However, a decreasing trend in FVIII transgene expression has been reported, for reasons that are not fully ...
Pseudotyped AAV Vector-Mediated Gene Transfer in a Human Fetal Trachea Xenograft Model: Implications for In Utero Gene Therapy for Cystic Fibrosis Lung disease including airway infection and inflammation currently causes the majority of morbidities and mortalities associated with cystic fibrosis (CF),......