We have created a matching set of shuttle plasmids that allow a one-step transfer of an entire expression cassette between the backbones of AVVs and LVVs. This has permitted a fair assessment of the impact of the vector type on tropism for both AVVs and LVVs. Thus, the aims of this ...
The size of the eCas9.4NLS expression unit (i.e.,∼6.5 kb; Fig.1a), exceeds the effective packaging capacity of commonly used viral vectors, such as lentiviral and adeno-associated viral vectors [27]. Hence, to achieve efficient delivery of transcriptional units encoding optimized Cas9 nu...
Each of these proteins has been engineered to alter the biological properties of the Ad vector, including tissue tropism, immunogenicity, and cell type specificity55. This capacity to alter the vector could result in next-generation vaccines with improved properties such as 1) the ability to avoid...
Homologous prime–boost regimens induced high levels of anti-vector neutralizing antibodies in Ad5-immunized mice, whereas the VSV-G-pseudotyped VRX1023 LV elicited low levels of anti-lentiviral vector neutralization. In addition, the heterologous prime–boost strategy resulted in a 5-fold reduction ...
Influence of short-term adenoviral vector and prolonged lentiviral vector mediated bone morphogenetic protein-2 expression on the quality of bone repair in a rat femoral defect model. Bone. 2008;42:921e31.Virk MS,Conduah A,Park SH, et al.Influence of short-term adenoviral vector and prolonged...
Sakoda, T, Kasahara, N, Kedes, L, Ohyanagi, M (2007) Lentiviral vector-mediated gene transfer to endotherial cells compared with adenoviral and retroviral vectors. Prep. Biochem. Biotechnol. 37: pp. 1-11Sakoda,T. et al.Lentiviral vector-mediated gene transfer to endotherial cells ...
Furthermore, transduction by lentiviral vectors had no significant effect on viability of BAECs. Our results indicate that lentivirus showed high‐level and long term gene expression in BAECs. Lentivirus can be an effective vector for the ex vivo, genetically modified EC implantation and in vivo ...
Virk MS,Conduah A,Park SH, et al.Influence of short-term adenoviral vector and prolonged lentiviral vector mediated bone morphogenetic protein-2 expression on the quality of bone repair in a rat femoral defect model.Bone. 2008Virk MS,Conduah A,Park SH. Influence of short-term adenoviral ...
Influence of short -term adenoviral vector and prolonged lentiviral vector mediated bone morphogenetic protein-2 expression on the quality ofbonerepair in a rat femoral defect model. Bone. 2008;42(5):921-931.Virk MS,Conduah A,Park SH, et al.Influence of short-term adenoviral vec...
in the French trial of gene therapy for X-linked severe combined immunodeficiency syndrome (SCID-X1) highlighted an issue of insertional mutagenesis of cancer-related host genes caused by random-integration into host chromosomal DNA of current vector systems, such as retroviral and lentiviral vectors....