Lentiviral vectors(LVs), derived from human immunodeficiency virus, are powerful tools for modifying the genes of eukaryotic cells such as hematopoietic stem cells and neural cells. With the extensive and in-depth studies on this gene therapy vehicle over the past two decades, LVs have been ...
36]. The discovery of eRNA is of great importance for the identification of enhancers and the in-depth study of functional mechanisms, while how eRNA affects enhancer function and the impact of eRNA on disease
Drug-induced liver injury (DILI) refers to drug-mediated damage to the structure and function of the liver, ranging from mild elevation of liver enzymes to severe hepatic insufficiency, and in some cases, progressing to liver failure. The mechanisms and clinical symptoms of DILI are diverse due ...
Another mitigation strategy involves incomplete CAR transduction during the manufacturing of infused CAR-T cells. The dosage of commercialized CAR-T-cell products correlates with the number of viable CAR-expressing (“CAR-positive”) cells. γ-Retroviral and lentiviral manufacturing methods yield ...
The genomes of certain viruses, such as lentiviruses, are modified to integrate exogenous genes into the host chromosome, thus causing stable protein expression. Infection with packaged viruses causes persistent transgene expression. Lentiviral vectors generally consist of two components, the packaging compo...
If safety, efficacy and improved quality of life can be demonstrated, these therapies have the potential to be the new standard of care treatments for LSD patients. 展开 关键词: AAV blood–brain barrier gene therapy lentiviral vectors lysosomal storage disorders mucopolysaccharidosis stem cells ...
Key studies for RNA structure and function particularly for promising RNA therapeutics such as vaccines, retroviral and lentiviral vectors, aptamers, anti-... MA Pulido,J Luo,S Questa,... 被引量: 0发表: 2021年 RNA Structure Determination by Structural Probing and Mass Spectrometry: MS3D Recent...
Merten OW, Hebben M, Bovolenta C. Production of lentiviral vectors. Mol Ther Methods Clin Dev. 2016;3:16017. ArticlePubMedPubMed CentralCASGoogle Scholar Blaese RM, Culver KW, Miller AD, Carter CS, Fleisher T, Clerici M, Shearer G, Chang L, Chiang Y, Tolstoshev P, et al. T lymp...
Biosafety features of lentiviral vectors. Hum Gene Ther. 2013;24(2):132–42. 123. Chicaybam L, Abdo L, Bonamino MH. Generation of CAR+ T Lympho‑ cytes Using the Sleeping Beauty Transposon System. Methods Mol Biol. 2020;2086:131–7. 124. Deniger DC, Pasetto A, Tran E, Parkhurst...
Targeted delivery of immunomodulators to immune cells or immune organs.aLymph node targeting of nanovaccine.bIn situ DC targeting of antigen, cytokine, and adjuvant.cT cell targeting of lentiviral transfection Full size image In addition to improving LN targeting, bioorthogonal chemistry can be employed...