Lentiviral vectors(LVs), derived from human immunodeficiency virus, are powerful tools for modifying the genes of eukaryotic cells such as hematopoietic stem cells and neural cells. With the extensive and in-depth studies on this gene therapy vehicle over the past two decades, LVs have been ...
Lentiviral vectors. and discusses recent advances that improve their biosafety and demonstrate their potential for gene delivery into important target tissues of gene therapy. Retr... L Naldini,IM Verma - 《Advances in Virus Research》 被引量: 122发表: 2000年 Gene Therapy Progress and Prospects: ...
10 These ADA-corrected T cells were capable of responding to novel antigens and represented a new polyclonal T-cell repertoire. For another immunodeficiency, Wiskott–Aldrich Syndrome, T cells were also functionally corrected in vitro by transduction with lentiviral vectors (LVs) encoding the Wiskott...
Additionally, integration-deficient lentiviral vectors (IDLVs) have recently been reported to transduce motor neurons efficiently and permanently after intrauterine injection, indicating the potential for IDLVs to become effective tools to treat inherited neurodegenerative diseases171. 5.4. Gene therapy in ...
Merten OW, Hebben M, Bovolenta C. Production of lentiviral vectors. Mol Ther Methods Clin Dev. 2016;3:16017. Blaese RM, Culver KW, Miller AD, Carter CS, Fleisher T, Clerici M, Shearer G, Chang L, Chiang Y, Tolstoshev P, et al. T lymphocyte-directed gene therapy for ADA–SCID:...
The genomes of certain viruses, such as lentiviruses, are modified to integrate exogenous genes into the host chromosome, thus causing stable protein expression. Infection with packaged viruses causes persistent transgene expression. Lentiviral vectors generally consist of two components, the packaging compo...
To accomplish a substantial impact as an anti-tumor vaccine, however, additional enhancements in the specificity and transduction efficacy of gene transfer by recombinant AAV vectors to DCs are necessary [53,54]. There has been a lot of interest in rAAVs as a possible vaccine because of their...
Mesenchymal stem cells have a natural tropism for tumours and their metastases, and are also considered immunoprivileged. This remarkable combination of properties has formed the basis for many studies investigating their potential as tumour-specific del
However, a relative clonal dominance was detected in a patient with β-thalassemia Major, two years after the subject received genetically modified hematopoietic stem cells using lentiviral vectors. This disappointing result of the recent clinical trial using lentiviral vector tells us that the current ...
r. habib,et al. lentiviral vector-mediated survivin shrna delivery in gastric cancer cell lines significantly inhibits cell proliferation and tumor growth oncol. rep., 34 (2) (2015), pp. 859-867 crossref view in scopus google scholar [150] x.j. qin,et al. effect of lentivirus-mediated ...