( 2002 ) Production of lentiviral vectors . Curr Top Microbiol Immunol 261 , 107 – 121 .Zufferey R. Production of lentiviral vectors. Curr Top Microbiol Immunol. 2002;261:107-121.Zufferey, R. (2002) Production of lentiviral vectors. Curr. Top. Microbiol. Immunol . 261 , 107–121....
(2011). Production of lentiviral vectors in protein- free media. Current Protocols in Cell Biology, 50(1), 26.8.1--26.8.13.Kuroda H, Marino MP, Kutner RH, Reiser J (2011) Production of lentiviral vectors in protein-free media. Curr Protoc Cell Biol Chapter 26: Unit 26.28....
The interest in lentiviral vectors as tools for gene therapy has increased dramatically over the last few years. The number of published studies on lentiviral vectors reflects this growing interest. Whereas only four papers were published in 1996, more than 40 publications appeared in 2000. The vas...
of lentiviral vector production for scale-up in fixed-bed bioreactor AJ Valkama1,2,3,6, HM Leinonen2,3,6, EM Lipponen2,3,4, V Turkki1,2,3, J Malinen1,2,3, T Heikura1, S Ylä-Herttuala1,5 and HP Lesch1,2,3,4 Lentiviral vectors (LVs) are promising tools for gene therapy...
Production of lentiviral vectors in suspension cells using low proportion of supercoiled circular plasmid DNA Xin-An Lu, Ting He, Zhihai Han, Yanping Ding, Liang Zhao, Guanghua Liu, Floris De Smet, Xiaojun Huang, Danqing Chen, Feifei Qi, Xiangyu Zhao ...
Large Scale Transient Transfection for the Production of Lentiviral Vectors using the HYPERStack Vessel Learn how the HYPERStack vessel can produce a superior LV titer compared to traditional stacked layer cell culture vessels. Learn More Large-scale Production of Influenza A Virus Using HYPERFlask Vesse...
Production of lentiviral vectors in a 96-well format is a common starting point for a variety of high-throughput applications, including arrayed CRISPR or shRNA-based screening and vector optimization workflows. However, obtaining sufficient viral titers in a high-throughput context...
These data highlight the positive impact of using our complete system to maximize production of lentiviral vectors compared to titers obtained when using incomplete or non-optimized systems(Figure 3). Lentivirus was produced in 30 mL shaker flasks using our LV-MAX Lentiviral Production Sy...
Figure 3. Lentivirus titers are dramatically improved using LV-MAX Lentiviral Production System. Cost effective Unfiltered lentivirus produced by suspension cells using the LV-MAX Lentiviral Production System was compared with polyethylenimine (PEI)–mediated transfection of lentiviral vectors in adh...
Lentiviral vectors are widely used in the field of gene therapy as an effective method for permanent gene delivery. While current methods of producing small scale vector batches for research purposes depend largely on culture flasks, the emergence and popularity of lentiviral vectors in translational,...