Naldini and Cantore’s goal is to use lentiviral vectors for liver-directed gene therapy of hemophilia, the coagulation disorder. “Since lentiviral vectors can stably integrate into target cells, the approach could enhance treatment prospects in younger patients whose livers are still growing,” expl...
The Advent of Lentiviral Vectors-Chapter 6:Prospects for Cancer TherapyMichel SadelainIsabelle Rivière
Recombinant lentiviral vectors used in the study.Marguerite KreitDidier VertommenLaurent GilletThomas Michiels
In a Perspective, [Amado and Chen][1] discuss the new generation of vectors that are based on lentiviruses such as HIV. They discuss the advantages of such lentiviral vectors but also raise the safety concerns inherent in using such vectors and how these can be overcome. [1]: http://www...
It also publishes Progress & Prospects reviews and News and Commentary articles, which highlight the cutting edge of the field.doi:10.1038/sj.gt.3302450A W NienhuisGene TherapyNienhuis AW.Lentiviral vectors for the treatment of Wiskott-Aldrich syndrome. Gene Therapy . 2005...
For lentiviral vectors containing the fluorescent marker ZsGreen, including the pooled gRNA library, the titration was also performed in primary CD4+ T cells, and the percentage of cells expressing ZsGreen was quantified 2 to 3 days post-transduction by flow cytometry using CytoFLEX S (Beckman) ...
High titers of lentiviral vectors are required for the efficient transduction of a gene of interest. During preparation of lentiviral the vectors, the protein of interest is inevitably expressed in the viral vector-producing cells. This expression may affect the production of the lentiviral vector. ...
(CAR-iMACs) by transducing CAR into induced human pluripotent stem cells (ihPSCs) or researchers developed CCR7-targeted CAR M by lentiviral transducing. Adenovirus is another vehicle for macrophages that have been utilized as viral vectors. The high expression of CD46 in macrophage surfaces ...
(gang.zhang@ utoronto.ca or sdzbzhanggang@ yahoo.com) Quantitative assessment on the cloning efficiencies of lentiviral transfer vectors with a unique clone site Gang Zhang & Anurag Tandon Department of Medicine, Centre for Research in Neurodegenerative Diseases, University of Toronto, 6 Queen's ...
Here, we generated a set of lentiviral vectors (with internal CMV or PGK promoter) in which we inserted (at the level of SIN-LTRs) sequences of avian (i.e., chicken hypersensitive site-4, cHS4), human (i.e., putative insulator and desert sequence), or bacterial origin. We ...