“CDC48A, an interactor ofWOX2, is required for embryonic patterningin Arabidopsis thaliana”文中，WOX2是一个已知的调节植物胚胎发育相关的重要基因，通过IP-MS找WOX2潜在互作蛋白，其中CDC48A因为在之前的研究中被证明也与胚胎发育相关，因此被作为了重点关注的蛋白，然后通过BiFC和Co-IP进行下游验证。从以上使...
ENHANCED DISEASE RESISTANCE4 associates with CLATHRIN HEAVY CHAIN2 and modulates plant immunity by regulating relocation of EDR1 in Arabidopsis[J]. The Plant Cell, 2015, 27(3): 857-873. 好文推荐: Co-IP与信号通路研究 酵母筛库存在自激活怎么办? 也许你离高分文章就差一个Co-IP! 【小远答疑】...
3. Wu G, 等. ENHANCED DISEASE RESISTANCE4... in Arabidopsis[J]. The Plant Cell, 2015.更多Co-IP和IP-MS实验技术，小远生物提供一站式服务，包括载体构建、Co-IP和IP-MS实验等。
When optimized to work with plant material, it proved to be suitable for the isolation of different types of nuclear complexes from Arabidopsis. Here, we describe a detailed protocol for the isolation of chromatin remodeling and polycomb repressive complexes using Arabidopsis lines that express GFP-...
Wu G, Liu S, Zhao Y, et al. ENHANCED DISEASE RESISTANCE4 associates with CLATHRIN HEAVY CHAIN2 and modulates plant immunity by regulating relocation of EDR1 in Arabidopsis[J].The Plant Cell, 2015, 27(3): 857-873. 向上滑动查看更多内容...
Wu G, Liu S, Zhao Y, et al. ENHANCED DISEASE RESISTANCE4 associates with CLATHRIN HEAVY CHAIN2 and modulates plant immunity by regulating relocation of EDR1 in Arabidopsis[J]. The Plant Cell, 2015, 27(3): 857-873. 向上滑动查看更多内容 ...
Wu G, Liu S, Zhao Y, et al. ENHANCED DISEASE RESISTANCE4 associates with CLATHRIN HEAVY CHAIN2 and modulates plant immunity by regulating relocation of EDR1 in Arabidopsis[J]. The Plant Cell, 2015, 27(3): 857-873. 向上滑动查看更多内容 ...
Wu G, Liu S, Zhao Y, et al. ENHANCED DISEASE RESISTANCE4 associates with CLATHRIN HEAVY CHAIN2 and modulates plant immunity by regulating relocation of EDR1 in Arabidopsis[J]. The Plant Cell, 2015, 27(3): 857-873. 向上滑动查看更多内容 ...
In this chapter, we present a highly sensitive protocol for the immunoprecipitation of nuclear chromatin-linked proteins in Arabidopsis thaliana that does not rely on time-consuming nuclei extraction. Interaction partners are identified using mass spectrometry and confirmed by co-immunoprecipitation. To ...
ArabidopsisImmunoprecipitationTandem mass spectrometryProtein complex identificationGFPIndividual proteins often function as part of a protein complex. The identification of interacting proteins is therefore vital to understand the biological role and function of the studied protein. Here we describe a method ...