Describes the ARAMEMNON, a specialized database for Arabidopsis membrane proteins. Facilitation of the interpretation of gene and protein sequence data; Classification of transmembrane (TM) candidate proteins; Comparison between TM computation programs and the predictions of subcellular localization by signal...
At least 30 % of the genes in the database are associated with multiple mutants or mutants that have multiple names (Fig.2). These have been compiled in the database to reduce confusion in searches for information. Overall, this database can provide a tool for exploring the relationships be...
ARAMEMNON, a Novel Database for Arabidopsis Integral Membrane Proteins A specialized database (DB) for Arabidopsis membrane proteins, ARAMEMNON, was designed that facilitates the interpretation of gene and protein sequence dat... R Schwacke,A Schneider,EVD Graaff,... - 《Plant Physiology》 被引量...
Imaging of elongating tobacco (Nicotiana tabacum) pollen tubes transiently transformed with a PIP5K4-green fluorescent protein fusion construct revealed that the protein localized to the plasma membrane, particularly in the subapical region. Overexpression of PIP5K4-GFP delocalized the protein to the ...
(2005), which may, in part, be due to different methods of protein extraction. Fig. 2 The cellular component classification of identified Arabidopsis pollen proteins using gene ontology database at TAIR (http://www.arabidopsis.org/). The percentage values indicate the proportion of total ...
(Fig. 5a,b;Supplementary Fig. 15). Assuming equal DNA content, these data support a role ofMAIL1in condensation of pericentromeric heterochromatin. MAIL1 is a nuclear protein24, and analysis of a MAIL1-GFP fusion protein suggested that MAIL1 is not preferentially associated with chromocenters ...
protein (GFP)-tagged actin-binding domain (fABD2). As shown, the phosphomimic derivatives ADF4S105D-RFP and ADF4S106D-RFP did not co-localize with fABD2, while ADF4S6D-RFPhad attenuated association (Fig.2band Supplementary Fig.5). These data support a key role for these three serine ...
Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ: Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 1997, 25 (17): 3389-3402. Article PubMed CAS PubMed Central Google Scholar Kawai J, Shinagawa A, Shibata...
Detailed studies on AtTLP3 demonstrated that AtTLP3 is a PM-tethered PIP2 binding protein which functions redundantly with AtTLP9 in abscisic acid (ABA)- and osmotic stress-mediated seed germination. Our results suggest that AtTLPs possibly work in multiple physiological and developmental processes...
The protein was detected on a 10% SDS–PAGE and transferred to Immobilon NC Transfer Membrane (HATF00010, Millipore) to perform immunoblotting. TCP22 phosphorylation in the presence (WT) or absence of PPKs (ppk124) in response to the blue light (30 μmol m−2 s−1) was performed ...