“CDC48A, an interactor ofWOX2, is required for embryonic patterningin Arabidopsis thaliana”文中，WOX2是一个已知的调节植物胚胎发育相关的重要基因，通过IP-MS找WOX2潜在互作蛋白，其中CDC48A因为在之前的研究中被证明也与胚胎发育相关，因此被作为了重点关注的蛋白，然后通过BiFC和Co-IP进行下游验证。从以上使...
2018年12月4日，朱健康教授团队在PNAS上发表了题为：Leucine-rich repeat extensin proteins regulate plant salt tolerance in Arabidopsis的研究论文，该研究发现特异性细胞壁结构蛋白（LRXs），小肽（RALFs）和质膜定位受体样激酶（FER）可作为协调细胞壁完整性、植物生长和盐胁迫反应的信号通路。在该论文中，作者为...
5.做IP/MS实验时,为什么不能跑开条带只送有一条WB条带的去做质谱,而是全部条带打包送质谱? IP/MS是寻找与目的蛋白互作的潜在蛋白,与酵母双杂筛库的作用差不多,送只有一条WB条带去做实验就相当于酵母双杂点对点验证,并不是筛库,因此,需要将所有条带打包送去做实验。 6. 自己做完免疫共沉淀之后,送样进行质...
2018年12月4日,朱健康教授团队在PNAS上发表了题为:Leucine-rich repeat extensin proteins regulate plant salt tolerance inArabidopsis的研究论文,该研究发现特异性细胞壁结构蛋白(LRXs),小肽(RALFs)和质膜定位受体样激酶(FER)可作为协调细胞壁完整性、植物生长和盐胁迫反应的信号通路。在该论文中,作者为了研究LRX蛋...
P A, 等. An aphid effector targets trafficking protein VPS52... [J]. Plant physiology, 2017.3. Wu G, 等. ENHANCED DISEASE RESISTANCE4... in Arabidopsis[J]. The Plant Cell, 2015.更多Co-IP和IP-MS实验技术，小远生物提供一站式服务，包括载体构建、Co-IP和IP-MS实验等。
2018年12月4日,朱健康教授团队在PNAS上发表了题为:Leucine-rich repeat extensin proteins regulate plant salt tolerance in Arabidopsis的研究论文,该研究发现特异性细胞壁结构蛋白(LRXs),小肽(RALFs)和质膜定位受体样激酶(FER)可作为协调细胞壁完整性、植物生长和盐胁迫反应的信号通路。在该论文中,作者为了研究LRX...
When optimized to work with plant material, it proved to be suitable for the isolation of different types of nuclear complexes from Arabidopsis. Here, we describe a detailed protocol for the isolation of chromatin remodeling and polycomb repressive complexes using Arabidopsis lines that express GFP-...
Wu G, Liu S, Zhao Y, et al. ENHANCED DISEASE RESISTANCE4 associates with CLATHRIN HEAVY CHAIN2 and modulates plant immunity by regulating relocation of EDR1 in Arabidopsis[J].The Plant Cell, 2015, 27(3): 857-873. 向上滑动查看更多内容...
Adult plants of the WT and IpLEA OX lines were also used to investigate the effect of the overexpression of IpLEA in Arabidopsis. Seven-day-old Arabidopsis seedlings were transplanted into well-watered vermiculite with nutrient solution from MS medium plates. Until 30 days of age (water was wi...
Absence of SUN1 and SUN2 proteins in Arabidopsis thaliana leads to a delay in meiotic progression and defects in synapsis and recombination. Plant J. 2015;81:329–46. Article CAS PubMed Google Scholar Chen Y, Wang Y, Chen J, Zuo W, Fan Y, Huang S, Liu Y, Chen G, Li Q, Li J...