AP-MS步骤:1.将标签和诱饵蛋白融合表达,形成载体;2.在目标细胞中过表达带有标签的诱饵蛋白;3.将细胞裂解液与磁珠孵育;4.洗脱得到蛋白互作复合物;5. LC-MS/MS检测复合物。 二、优缺点 三、适用范围 IP-MS: 适用于鉴定已知蛋白质的潜在相互作用蛋白。 适用于研究在天然生理条件下蛋白质的相互作用。 常用于验证...
3. KEILHAUER E C, HEIN M Y, MANN M 2015. Accurate protein complex retrieval by affinity enrichment mass spectrometry (AE-MS) rather than affinity purification mass spectrometry (AP-MS). Mol Cell Proteomics [J], 14: 120-135. 4. MELLACHERUVU D, WRIGHT Z, COUZENS A L, et al. 2013. T...
3. KEILHAUER E C, HEIN M Y, MANN M 2015. Accurate protein complex retrieval by affinity enrichment mass spectrometry (AE-MS) rather than affinity purification mass spectrometry (AP-MS). Mol Cell Proteomics [J], 14: 120-135. 4. MELLACHERUVU D, WRIGHT Z, COUZENS A L, et al. 2013. T...
3. KEILHAUER E C, HEIN M Y, MANN M 2015. Accurate protein complex retrieval by affinity enrichment mass spectrometry (AE-MS) rather than affinity purification mass spectrometry (AP-MS). Mol Cell Proteomics [J], 14: 120-135. 4. MELLACHERUVU D, WRIGHT Z, COUZENS A L, et al. 2013. T...
Accurate protein complex retrieval by affinity enrichment mass spectrometry (AE-MS) rather than affinity purification mass spectrometry (AP-MS). Mol Cell Proteomics [J], 14: 120-135. MELLACHERUVU D, WRIGHT Z, COUZENS A L, et al. 2013. The CRAPome: a contaminant repository for affinity ...
3. KEILHAUER E C, HEIN M Y, MANN M 2015. Accurate protein complex retrieval by affinity enrichment mass spectrometry (AE-MS) rather than affinity purification mass spectrometry (AP-MS). Mol Cell Proteomics [J], 14: 120-135. 4. MELLACHERUVU D, WRIGHT Z, COUZENS A L, et al. 2013. ...
Kit for IP/ Co-IP of Spot-tagged proteins & sample preparation for mass spectrometry (MS) Specificity Spot-Tag® Applications IP, MS Type Nanobody Conjugate AgaroseCat no : etak-iST Synonyms Spot tag, Spot, PDRVRAVSHWSS, Spot-Trap
Kit for IP/ Co-IP of GFP-fusion proteins & sample preparation for mass spectrometry (MS) Specificity GFP and GFP derivates Applications IP, MS Type Nanobody Conjugate Agarose Publications 3 Cat no :gtak-iST Synonyms GFP, eGFP, eYFP, CFP, YFP, BFP, GFP-Trap, Green fluorescent...
APMAP在肿瘤中的表达明显增高,说明APMAP可作为前列腺癌的潜在诊断标记物。 结论: 先前的研究发现胆固醇会增加侵袭性前列腺癌的患病风险,本项目研究进一步证明胆固介导APMAP上调,进而抑制EGFR降解,激活ERK1/2通路,从而促进前列腺癌细胞EMT;提出APMAP可能是一种关键的调节剂,为高脂肪饮食、肥胖和癌症转移之间提供了联系。
本研究证明胆固醇介导APMAP上调,APMAP通过与EGFR竞争结合EPS15R,进而抑制EGFR降解,激活ERK1/2通路,促进前列腺癌细胞EMT;提出APMAP可能是一种关键的调节剂,为高脂肪饮食、肥胖和癌症转移之间提供了联系。 研究路线 细胞功能实验证实胆醇诱导前列腺癌细胞EMT的发生依赖于ERK1/2的激活RNA-seq、蛋白组学等实验筛选和验证...