Firstly, we measured S100A8 from 78 bone marrow (BM) plasmas, including 50 acute myeloid leukemia (AML) patients, myeloproliferative neoplasms (MPN), myelodysplastic syndrome (MDS), and healthy donors (HD). (D) Correlation of BM [S100A8] with the percentage of leukemic cells in BM, ...
In conclusion, elevated BM plasma levels of sCD30 reflected the enhanced CD30+ T cell-mediated immune response in SAA. CD30 as a molecular marker that transiently expresses on IFN-γ-producing T cells, may participate in mediating bone marrow failure in AA, which also can facilitate our ...
The differentiation of osteoblasts from mesenchymal stem cells or osteoprogenitor cells in bone marrow is mainly regulated by BMP-2, a transforming growth factor-β (TGF-β) originally described as a bone morphogens, that enhances ALP activity in a pluripotent mesenchymal precursor cell line, C2C1216...
Hydroxyapatite (HA) granules are frequently used in orthopedics and maxillofacial surgeries to fill bone defects and stimulate the regeneration process. Optimal HA granules should have high biocompatibility, high microporosity and/or mesoporosity, and high specific surface area (SSA), which are essential...
Bone marrow cells (BMCs) from femoral bones were kept in growth medium for differentiation to macrophages. We used F4/80 as the marker for mouse macrophages to validate the differentiation of macrophages. To study effects of muMPO ANCA on CD206 and MPO expression in macrophages, BMDMs were ...
Purified bone marrow cells irradiated to 1500 cGy and serial dilutions were prepared starting at 50 000 cells/well to 400 cells/well in 96-well culture plates containing RPMI media with 50 ng/mL IL-3, 100 ng/mL GM-CSF, and 10% AB− human plasma. Then 50 000 allogeneic peripheral ...
Multiple myeloma (MM) is a clonal B-cell disorder of terminally differentiated plasma cells that accounts for ≈ 10% of hematologic malignancies.1, 2 It is the second most common hematologic malignancy in the United States, with an overall incidence rate of 4.4 cases per 100 000 population/...
Percoll-enriched DR+ PBMC were adjusted to 1 × 106 cells/mL and T-cell-enriched PBMC were adjusted to 4 × 106 cells/mL; 50 μL of each cell suspension was added to each well (final ratio T:DR+, 4:1). The plates were then incubated for another 24 hours at 37°C in a 5% ...
In addition, hematocrit, hemoglobin, plasma proteins and cortisol values, as well as norepinephrine/epinephrine and serotonin concentration were determined. Puppies treated with CT + I showed 81% survival, mild clinical signs, and a significant decrease in circulating neutrophils and lymphocytes in...
Elimination of acute myelogenous leukemic cells from marrow and tumor suspensions in the rat with 4-hydroperoxycyclophosphamide. Blood. 1980;55:521–3. Article CAS PubMed Google Scholar Gorin N, Aegerter P, Auvert B, Meloni G, Goldstone A, Burnett A, et al. Autologous bone marrow ...