A quantitative, sensitive, One-Step In Situ hybridization assay is provided which will detect as few as 1-5 copies of target biopolymer per cell and may be accomplished in 5 minutes to 4 hours. There is provided a simultaneous assay for detecting multiple biopolymers within the same cell....
Proceed to the denaturation and hybridization steps. You should then add the probe, hybridize overnight, and perform the detection as usual. Need more information? Contact us › For Research Use Only. Not for use in diagnostic procedures....
The protocol has been optimized for the detection of DNA in the positive control slides provided with the in situ hybridization and detection kit. This general method may need further optimization in the pretreatment and hybridization steps. Pretreatment 1. Label the side of the slide containing the...
Whole-mount in situ hybridization is a powerful technique that enables scientists to understand the molecular basis of embryonic development. "Whole-mount" indicates that the entire embryo will be used, not just a tissue slice. "In situ" is a Latin phrase meaning "in position." And finally, ...
The HybEZ™ Hybridization System maintains optimum humidity and temperature during the assay workflow and is required for RNAscope™ hybridization steps. Superfrost®Plus slides are required. Other slide types may result in tissue detachment. ...
Specialized microorganisms catalyse central steps of global element cycling, such as nitrogen fixation or the mineralization of organic matter. There is an urgent need for the development of new methods forin situmicrobial analysis, which originates from the restricted morphological diversity of prokaryote...
Products for Fluorescence In Situ Hybridization (FISH) Tyramide Signal Amplification (TSA) Introduction This protocol describes steps for fluorescent in situ hybridization (FISH) to Drosophila embryos using Tyramide Signal Amplification (TSA™), and was developed in collaboration ...
When performing in situ hybridization for the first time, it is often desirable to monitor cytoplasmic RNA loss during the procedure by staining the slides with propidium iodide during the steps in the procedure. II. Fixation Mammalian cells are grown directly onto coverslips, washed once in 1X ...
For in situ hybridization, slides were then submerged in 50% ethanol, 70% ethanol, and two 100% ethanol steps for 5 min each at room temperature. Slides were then pretreated with solution according to the manufacturer directions for 30 min, and then washed with 1xPBS twice. The probe...
Guidelines for RNA probe preparation, dissection of soft tissues and the general procedure for fixation and hybridization are described in Part A, while steps specific for the collection, fixation, pre-hybridization and hybridization of mosquito embryos are detailed in Part B. 展开 ...