The higher sensitivity of the hybridization test compared to culturing could mainly be explained by the failure to detect haemophilus in culture caused by masking due to overgrowth by other bacteria. In conclusion the in situ hybridization test, which can be performed in only 4 h, is a ...
RNA in situ hybridization 18-DAP seeds from the middle region of the ears were harvested and fixed in 4% paraformaldehyde solution with 0.1% Triton X-100 and 0.1% Tween 20 in phosphate-buffered saline (PBS) (Takara, Cat# T900) overnight. The seeds were dehydrated using a gradient concentra...
, in situ hybridization, microarray, and small RNA sequencing. Accordingly, circulating miRNAs are attractive and promising biomarkers for clinical applications in RA, such as aiding early diagnosis, monitoring of the disease course, and prediction of treatment response. Contribution to prevention and pr...
Massé et al., “Optimization of the fluorescence in situ hybridization (FISH) technique for high detection efficiency of very small proportions of target interphase nuclei - Yan, Guilbault, et al. - 2000 () Citation Context ...e explained by chromatin extension forming chromatin fibers, which ...
Imaging techniques, such as fluorescent in-situ hybridization (FISH), can also show where specific opsins are expressed across the retina. Genetic evidence suggests reef fishes, like other fishes, may select spectral sensitivities from a repertoire of visual genes (opsins), and this can occur on ...
(GW 23) human brain tissue by jointly imaging the 3D organization, gene expression and nuclear architectural proteins using the chromatin tracing and RNA multiplexed error-robust fluorescence in situ hybridization (MERFISH) platforms30,31,32. Specifically, the median-sized chromosome 14 was imaged ...
In situ hybridization of 3′ UT directed antisense oligomer probe demonstrates diffuse localization of Ap3d transcripts in forebrain of wild-type (A) and mh/mh (B) gray matter regions, with highest levels of mRNAs in the granule cell layer of the dentate gyrus and hippocampal pyramidal cell la...
more than 97% of theSulfurimonassequences of these three remote sites on ultraslow spreading ridges belonged to two closely related operational taxonomic units (OTU1 and OTU2), with a similarity of 99.5%. Fluorescence in situ hybridization using both a Campylobacterota-specific rRNA probe and tailo...
For in situ hybridization, Axin2 probe was linearized by SallI and labeled using the DIG RNA Labeling Mix (Roche). Sections were rehydrated, refixed, bleached, digested with proteinase K and acetylated with acetic anhydride. Hybridization was performed o/n at 63 °C. Sections were blocked ...
(Fig.9b). To test if Pnt has a broader function in SOP lineages, we examined the expression of receptors in other sensillar classes in control andpntRNAi antennae by RNA in situ hybridization. In the four classes of two-OSN sensilla tested (ab2, ab3, ai2, ac3), duplication of the ...