This paper describes methods that are commonly used for performing mRNA in situ hybridizations. Each stage of the procedure has been analyzed to identify the parameters that most significantly affect the final cell morphology and sensitivity of the system. We have identified key elements of the ...
sections for the protocol to work properly. When smaller samples are utilized, such as zebrafish or sea urchin embryos, anin situcan be performed on an entire sample. Although the models used to perform this technique can be quite different, anin situhybridization procedure remains relatively the...
Quantitative aspects of anin situhybridization procedure for detecting mRNAs in cells using 96-well microplates H Zreiqat,R Sungaran,CR Howlett,... 被引量: 0发表: 1998年 In situ hybridization of astrocytes and neurons cultured in vitro When cells are cultured on specialized microplates, ISH can...
a procedure that allows researchers to locate the positions of specificDNAsequences on chromosomes. Since the firstin situhybridization experiments in 1969 (Gall & Pardue, 1969), many variations of the procedure have been developed, and its sensitivity has increased enormously. Today, mostin situhyb...
Add hybridization buffer to the rest of the probe such that it is at a final concentration somewhere in the range of 5–50 ng/µL. Store the probe stock at ≤–20°C until you are ready for the hybridization procedure. TOP 3. Hybridizing the Probe Place the ...
With the aim of optimizing in situ hybridization methods, alkaline, acid, and thermal denaturation procedures have been studied for their ability to separate the DNA strands of nuclear DNA and for the DNA losses they induce. Isolated methanol/acetic acid-fixed mouse liver nuclei have been used ...
(EBV). Most of these viruses, however, can be easily identified byimmunohistochemistry, which has become a routine procedure in most laboratories and provides a degree of sensitivity that is similar to that of in situ hybridization.61In situ hybridization (ISH) is particularly useful, however, ...
hybridization procedure. After rinsing with Reaction Buffer, sections were blocked against unspecific binding for 12 min at 37 °C (from DIG wash and Block buffer set, 11 585 762 001, Roche, Mannheim, Germany). For the immunologic detection, we used prediluted Alkaline phosphatase (AP)-...
专利名称：IN SITU HYBRIDIZATION SOLUTION AND PROCESS 发明人：KIM, In, C.,THOMPSON, Donald, M.,SARTO, Gloria, E.申请号：EP96915311.0 申请日：19960405 公开号：EP0820525A1 公开日：19980128 专利内容由知识产权出版社提供 摘要：A fluorescence in situ hybridization (FISH) procedure and solutions is ...
this chapter, we describe optimized fluorescent in situ hybridization protocols for Drosophila embryos and tissues utilizing tyramide signal amplification, either for single genes or in a high-throughput format, which greatly increases the sensitivity, consistency, economy, and throughput of the procedure....