ISH主要步骤包括:合成核酸探针、标记、纯化和特异性靶标退火(基本工作流程与印迹杂交近似),目前主要有两种办法来实现可视化显示原位RNA和DNA靶标,即,即荧光原位杂交 (Fluorescence In Situ Hybridization, FISH),显色原位杂交 (Chromogenic in situ hybridization, CISH) 检测和银染原位杂交(Silver-enhanced in situ ...
in situ hybridizationmicrotiter plateAt present, in situ hybridization (ISH) study of human papillomavirus (HPV) at the ultrastructural level is limited because of the complexity of the techniques. We have developed a method for electron microscopy ISH that is easily adapted for routine use. The ...
In Situ Hybridization Techniques A useful complement toqPCRis offered byin situ hybridizationfor the detection of viralnucleic acidswithin cells or tissues with preservation of cellular morphology. In the case of biopsies, the quantitative information offered by qPCR on purified nucleic acids should not...
I n situ hybridization techniques enable gene expression to be monitored in individual cells (Figure 18.1). Methods for use with the light microscope are as sensitive as northern blot analysis for detecting mRNAs that accumulate equally in all cells, and more sensitive than northern analysis for de...
Recently, in situ hybridization techniques with combinatorial encoding have emerged in which the identity of hundreds or thousands of RNA species can be decoded with tens of FISH cycles11,12. Although these methods have increased the multiplexity by 2-3 orders of magnitude compared to smFISH, they...
Amann, R., Fuchs, B. Single-cell identification in microbial communities by improved fluorescencein situhybridization techniques.Nat Rev Microbiol6, 339–348 (2008). https://doi.org/10.1038/nrmicro1888 Download citation Issue DateMay 2008
In situhybridization (ISH) is a technique to determine potential correlations between viruses and lesions. The aim of the study was to compare ISH techniques for the detection of various viruses in different tissues. Tested RNA viruses include atypical porcine pestivirus (APPV) in the cerebellum ...
Nonradioactive In Situ hybridization Application Manual, 2nd edition, 51–56 (Boehringer Manheim 1996). Friml, J., Benková, E., Mayer, U., Palme, K. & Muster, G. Automated whole-mount localization techniques for plant seedlings. Plant J. 34, 115–124 (2003). Article CAS Google Scholar...
Applications of in Situ Hybridization Techniques in the Diagnosis of Chronic Sinusitis: The clinical significance of positive bacterial cultures in chronic sinusitis is often difficult to assess. Contaminants from surface colonization of the s... Dr.,Peter,H.,... - 《American Journal of Rhinology...
In addition, several in situ hybridization techniques, such as proximity ligation in situ hybridization technology (PLISH) [82] and SCRINSHOT [83] have been developed and used for identifying and localizing lung and airway cell types in mice, including the recently discovered ionocytes. In the ...