a In situ hybridizations of anti-sense DIG-labeled riboprobes demonstrate that endogenous C1qL4 (right) is expressed in muscle cells that also express the myocyte differentiations Pax3 and MyoD (left and middle) at the indicated embryonic days of chicken embryo development. C1qL4 is also ...
For in situ hybridization, liveM. alaskensisspecimens were first relaxed using 1:1 0.37 M MgCl2:FSW for 10 min, followed by fixation overnight at 4 °C in 4% paraformaldehyde in FSW. All wash and incubation steps were 5–10 min, unless otherwise indicated. Fixation was followed ...
We first examined the expression oflncRNA-HSVIIIby making paraffin sections and performing in situ hybridization without amplification of signals using the TSA system. Unfortunately, no signal was detected by this method. We then examined the expression patterns of this lncRNA by combining paraffin s...
The alternative explanation, that intrastriatal stimuli better activate inhibition, was discarded in the present work: it was shown that polysynaptic activation of inhibitory entries is larger when the stimulus is within the cortex and not within the striatum. To conclude: accepting that cortical ...
clinico-pathological workflow and reflect the standard processing of tissue specimens in pathological routine diagnostics worldwide; (3) FFPE tissues are intrinsically linked to clinical records and often associated with additional pathological data (genomics, in situ hybridization, immunohistochemistry etc.)...
van Leen RW, Breuer ML, Lubsen NH, Schoenmakers JG: Developmental expression of crystallin genes: in situ hybridization reveals a differential localization of specific mRNAs. Dev Biol. 1987, 123: 338-345. 10.1016/0012-1606(87)90392-7. Article CAS PubMed Google Scholar van Leen RW, van Roo...
(F) RNA in situ hybridization of pan-MPE (HOXB13) and SP-Progenitor-like (JUNB) gene markers in FFPE tissue matched to scRNA-seq samples. Arrows and asterisks indicate representative cells positive for HOXB13, and JUNB, respectively. (G) Expression of Hoxb13 in the developing mouse embryo...
Hybridization was performed using Agilents In situ Hybridzation kit 51885242. Chips used for microarray were customized for M. smegmatis MC2 155815k Array AMADID: 020791 (Genotypic Technology, Bangalore, India). c) Transcriptome data analysis The raw data obtained from experiments have been ...
Here, we combine immunofluorescence and fluorescence in situ hybridization techniques to simultaneously visualize bunyavirus progeny virions and their genomic content at single-molecule resolution in the context of singly infected cells. Using Rift Valley fever virus and Schmallenberg virus as prototype ...
, in situ hybridization, microarray, and small RNA sequencing. Accordingly, circulating miRNAs are attractive and promising biomarkers for clinical applications in RA, such as aiding early diagnosis, monitoring of the disease course, and prediction of treatment response....