a–gCancer vaccination model of Mock or PTENα transfectedPten−/−B16 cells.aSchematic diagram of the cancer vaccine model.bTumor volumes of C57BL/6 mice subjected to the cancer vaccination model were monitored overtime. The unimmunized group refers to mice without irradiated B16 cells immuni...
γδ T cells are a distinct subgroup of T cells that bridge the innate and adaptive immune system and can attack cancer cells in an MHC-unrestricted manner. Trials of adoptive γδ T cell transfer in solid tumors have had limited success. Here, we show that DNA methyltransferase inhibitors ...
(A) Schematic diagram of CyTOF experiments. MC38 tumor cells were subcutaneously injected into C57BL/6 mice. Tumor tissues were collected on day 25 and digested into a single-cell suspension. CD45+ cells were isolated using magnetic beads and labeled for CyTOF analysis. n = 8 mice per...
Fluorescently labeled secondary antibodies (FITC, Rhodamine RX, and Cy5) were obtained from Jackson Immunoresearch (West Grove, PA). Immunofluoresence analysis and quantification Fluorescent immunostains were imaged using a Leica DMi8 microscope with a Leica DFC9000GT camera controlled by Leica ...
Intracellular cytokine expression was detected with anti-IFN-γ-APC or anti-TNF-γ-APC after cells were surface labeled and permeabilized. The results are reported as the percentage of cell type (geometric means with SEM) containing TNF-α or IFN-γ, and analysis of each spleen was determined...
After purification, the DNA fragment was used as the template for in vitro transcription to generate DIG-labeled RNA probes using the DIG Northern Starter Kit (Roche). Equal amounts of total RNAs collected from S2 cells transfected with different concentration of pMT-DCV-1A plasmids were ...
Gene loci are labeled by the gene name and corresponding Entrez geneID. A chromosome locus may contain more than one gene. Therefore IIDB allows multiple labels to be associated with a single locus. Locus coordinates were used to search the ENSEMBL database for repeats, CpG islands, and ...
Subsequently, a gene was labeled DIU provided that at least one of its isoforms showed significant evidence of differential usage between African-Americans and European-Americans. In particular, using this approach 93, 70, and 123 genes were detected with significant DIU (FDR < 0.05) between ...
Central nervous system IDH: Isocitrate dehydrogenase MGMT: O6-Methylguanine-DNA methyltransferase GSEA: Gene Set Enrichment Analysis GO: Gene Ontology HK3-OE: HK3 overexpression NC: Negative control PCR: Polymerase chain reaction qRT‒PCR: Quantitative real-time PCR IHC: Immunohistochemistry...
Epidemiological studies have suggested that the encounter with commensal microorganisms during the neonatal period is essential for normal development of the host immune system. Basic research involving gnotobiotic mice has demonstrated that colonization at the age of 5 weeks is too late to reconstitute...