Illumina sequencing and array technologies drive advances in life science research, translational and consumer genomics, and molecular diagnostics.
加入中和液与index primer 测序方法与前述相同,可以测得index序列,从而区分出该DNA片段来源于某个具体的样本 来源:illumina 2.3.4 双端测序 Paired-end Sequencing双端测序大大提升了某一个DNA片段的有效测序长度,步骤如下: 合成互补链 切断模板链与flowcell引物的连接,保留互补链 与Read1测序原理类似,加入不同的...
2. NAOH使双链变性为单链,并洗去已经测序完成的Forward strand 3. 类似的,readprimer2结合到靠近P7’的read primer binding site 2开始对Reverse strand的测序。测序完成后即可得到Reverse read序列。 前面介绍的都是paired-end的测序,而single-end测序方式是只将index,sequencing primer binding site以及P7/P5添加到...
1. 利⽤转座⼦（transposome）对双链DNA进⾏剪切以及接头（adapter）的连接 2. 接头连接成功后，利⽤低循环扩增技术在接头处进⾏修饰，分别在两端添加sequencing primer binding site1/sequencing primer binding site2（即测序引物结合位点）、index1/index2以及我们称之P5和P7的寡核苷酸序列 上图并没有将...
paried-end sequencing 一、Library Preparation文库的构建 1. 利用转座子(transposome)对双链DNA进行剪切以及接头(adapter)的连接 2. 接头连接成功后,利用低循环扩增技术在接头处进行修饰,分别在两端添加sequencing primer binding site1/sequencing primer binding site2(即测序引物结合位点)、index1/index2以及我们称...
–清楚了解Illumina测序流程中的主要步骤–能够描述簇生成（ClusterGeneration）的过程–理解边合成边测序（SequencingbySynthesis）的原理 2Part#15045845_Rev.C FORRESEARCHUSEONLY IlluminaSequencingWorkflowIllumina测序流程 LibraryPreparation文库制备 ClusterGeneration簇生成 cBotMiSeqNextSeqHiSeq2500-Rapid Sequencing测序 Data...
5、Sequencing 测序 Cluster Generation 簇生成 Library Preparation 文库制备测序流程13Hiseq2500Two flow cell1410. Sequencing primer is hybridized to adapter sequence测序引物3端5端1511. Only one nucleotide is added every cycle as the 3 end is blocked1612. Cleave the fluor and free the 3 end then ...
(~ 6 hrs)DNAcBotHiSeq25001-8 samplesMiSeqHiSeq2000HiSeq2500GA IIxMiSeq1-8 samplesHCS/RTAICSMSRCASAVABaseSpace 文构建流程片段化 DNA末端平3’加A接PCR 高量DNA文构Index SequencingPrimer此部分 与FlowCell表面上 接P7相同此部分 与FlowCell表面上P5接相同文构建的目的是在目的DNA片段两端都接上想要 的...
Dual-Indexed Workflow on a Paired-End Flow Cell4 Dual-Indexed Workflow on a Single-Read Flow Cell6 Sequencing Primers for HiSeq Systems8 Revision History9 Technical Assistance11 Document#15057455v03 ILLUMINA PROPRIETARY February2017 For Research Use Only.Not for use in diagnostic procedures.
uDual-indexedlibraries—Addsupto24unique8-baseIndex1(i7)sequencesandupto16unique8-base Index 2 (i5) sequences to generate up to 384 uniquely tagged libraries. Duringindexed sequencing, the index is sequenced in a separate read, calledthe Index Read, wherea new sequencing primer is annealed. ...