Illumina acquired Solexa, got the next-generation high-throughput sequencing technology and developed it into a mainstream technology on the market. It currently provides sequencing systems such as MiSeq, HiSeq 2500, HiSeq 3000, HiSeq 4000, HiSeq X Ten, HiSeq X five,...
NGS was originally developed from pyrophosphate sequencing principle. At present, more mature application platforms are various types of Ion Torrent, Illumina, and Complete Genomics (CG). Since its first appearance more than 10years ago, NGS technology had become increasingly mature. One of the ...
Overcoming bias and systematic errors in next generation sequencing data. Genome medicine. 2010; 2(12):87.74. Aird D, Ross MG, Chen WS, Danielsson M, Fennell T, Russ C, Jaffe DB, Nusbaum C and Gnirke A. Analyzing and minimizing PCR amplification bias in Illumina sequencing libraries. ...
TIANGEN biochemical library construction products can well match the requirements of the next generation sequencing platform, which include library construction kits, modules, raw materials and customized solutions for Illumina sequencing platform library construction. TIANGEN also provides customized library cons...
The next generation sequencing—NGS sequencing, has the core principle of sequencing while synthesizing or ligating. By randomly breaking template DNA into small fragments through physical or chemical methods and amplifying the library through PCR, hundreds of thousands to millions of DNA templates are ...
Steps of a sequencing experiment. Black arrows indicate steps that are common for all second-generation sequencing (SGS) technologies, white arrows refer to the Illumina systems, and grey arrows refer to the Roche 454 and SOLiD systems.
conversion -- for various Illumina platform-based bisulfite sequencing (bisulfite-seq) assays, such as whole genome bisulfite sequencing (WGBS), oxidative bisulfite sequencing (oxBs-seq), reduced representation bisulfite sequencing (RRBS), and other bisulfite-based next generation sequencing applications. ...
Next-generation sequencing as a tool for detailed molecular characterization of genomic insertions and flanking regions in genetically modified plants:a pilot study using a rice event unauthorized in the EU. WAHLER D,SCHAUSER L,BENDIEK J,et al. Food Analytical Methods . 2013...
Currently, there are two representative types of third third-generation sequencing technologies: single-molecule real-time sequencing (SMRT) and nanopore sequencing. SMRT sequencing relies on the principle of sequencing by synthesis and utilizes nanoscale zero-mode waveguide to achieve real-time sequencing...
Original research article © American College of Medical Genetics and Genomics Next-generation sequencing improves thalassemia carrier screening among premarital adults in a high prevalence population: the Dai nationality, China Jing He, MS1,2, Wenhui Song, PhD3, Jinlong Yang, BS3,5,...