The Illuminanext-generation sequencing (NGS)method is based on sequencing-by-synthesis (SBS), and reversible dye-terminators that enable the identification of single bases as they are introduced into DNA strands. Theoretically, Illumina NGS technology resemblesSanger sequencing. During the cyclic process...
Read lengths vary based on the platform and application but tend to be shorter, e.g., short sequences on the Illumina platform. Speed Relatively slow, sequencing small DNA segments sequentially, requiring step-by-step analysis. Relatively fast, capable of concurrently processing multiple samples wit...
Pipeline for the study of DDIs using transcriptome analysis Figure 1 shows the flowchart for transcriptome analysis of DDIs. First, assays for DDIs are selected that are suitable for RNA sequencing and phenotype readouts. Second, shared differentially expressed (DE) genes from treatment with the ...
Whole-exome Sequencing was performed with DNA from fibroblast cells. Enrichment was done with an Illumina Enrichment Kit (Nextera Rapid Capture Exome v1.2) and the respective libraries were sequenced on a NextSeq500 sequencer (Illumina, San Diego, USA). Alignment and variant calling was performed...
Illumina sequencingHuman identificationHuman identification has played a prominent role in forensic science for the past two decades. Identification based on unique genetic traits is driving the field. However, this may have limitations, for instance, for twins. Moreover, high-throughput sequencing ...
A new generation of sequencing technologies is revolutionizing molecular biology. Illumina's Solexa and Applied Biosystems' SOLiD generate gigabases of nuc... P Konrad,DJ Studholme - 《Briefings in Bioinformatics》 被引量: 355发表: 2010年 How I use hydroxyurea to treat young patients with sickle ...
The Illumina sequencing data was deposited on the European Nucleotide Archive (ENA) under the project accession number PRJEB55471. Acknowledgments Simone Priesnitz and Annett Michel are gratefully acknowledged for excellent technical assistance. Conflicts of Interest The authors declare no conflict of ...
quantified by fluorometric measurements using a Qubit dsDNA HS assay kit (Life Technologies, Camarillo, CA, USA) and finally pooled in equimolar amounts for sequencing on a Illumina MiSeq platform using Illumina MiSeq 500 cycle Kit_V2 chemistry (2 × 250 base PE; Illumina, San Diego, CA, ...
Here we will provide some example of the TBP application and will discuss its features and uses in comparison with the DNA sequencing-based methods. Keywords: food authentication; metabarcoding; TBP; genotyping 1. Introduction This contribution is inspired by few simple concepts that, applied to ...