We use various media and supplements for the various primary cells. Details can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/primary-cell-culture.html). Cumulative population doublings are calculated from the seeding density and harvesting density from each cult...
Cell culture media and supplements were purchased from Kurabo Industries (Osaka, Japan). Fenofibrate was a kind gift from Kaken Pharmaceutical (Tokyo, Japan). Fetal bovine serum (FBS) was purchased from Gibco, Invitrogen (Carlsbad, CA). Wortmannin was purchased from EMD Biosciences (La Jolla, CA...
Optimising culture conditions for HSVECs Different media were investigated to differentiate which allowed the greatest expansion of HSVECs. The endothelial based medium EBM™-2 or a 50:50 mixture of SFM® and EBM™-2 with optimal concentration of FCS, EGFS and heparin (Fig. 1a and b) ...
We use various media and supplements for the various primary cells. Details can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/primary-cell-culture.html). Primary cells are cells taken directly from living tissue (e.g., biopsy material) and established for...