Thus, it can be concluded that the effect of the used medium outweighed the effect of HUVEC co-culture, emphasizing the importance of the culture medium composition for hiPSC differentiation.Freyer, NoraGreuel, SelinaKnoespel, FannyStrahl, NadjaAmini, LeilaJacobs, FrankMonshouwer, MarioZeilinger, ...
HUVECs were cultured on six-well culture plates. The cells were pretreated with resveratrol (100 μM) for 1 h and then with ox-LDL (0.1 mg/ml) for 8 h. The cells were fixed with 4% paraformaldehyde for 15 min and washed twice. The cells were then incubated with an antibo...
by flow cytometry. The presence of TNF-α significantly decreased the expression of ZO-1. A similar tendency could be observed in the presence of AC in the culture media. A combination treatment of TNF-α and AC led to a lower expression level, compared to the control cells; however, the...