We use various media and supplements for the various primary cells. Details can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/primary-cell-culture.html). Cumulative population doublings are calculated from the seeding density and harvesting density from each cult...
Cell culture and reagents HUVECs were purchased from PromoCell (C-12200, Heidelberg, Germany) and cultured in EndoGRO-LS media (Millipore, MA, USA) as described [46]. EE and LE in HUVECs were labeled by CellLight EE/LE-GFP, BacMam 2.0 for 24 h before fixation or live imaging accor...
Differences were assessed by one-way ANOVA followed by Dunnett’s multiple comparison test. Results Cell viability Proteases from various sources are routinely used in cell culture to detach cells from surfaces of tissue culture flasks. At higher concentrations and/or prolonged incubation time proteases...
Bar graphs on the right panel of the figure present the mean ± SEM of three independent experiments relative to control where "a" represents statistical significance at p < 0.05 as compared to control (one-way ANOVA followed by Tukey's multiple comparison test) that the ferritin ...
We use various media and supplements for the various primary cells. Details can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/primary-cell-culture.html). Primary cells are cells taken directly from living tissue (e.g., biopsy material) and established for...
by flow cytometry. The presence of TNF-α significantly decreased the expression of ZO-1. A similar tendency could be observed in the presence of AC in the culture media. A combination treatment of TNF-α and AC led to a lower expression level, compared to the control cells; however, the...
(PBS) was then added to the wells and incubated for 1 h at 37 °C. Afterwards, culture media were added and incubated for another hour at 37 °C. HUVECs were seeded on the substrates at 1 × 104cells/cm2in serum-containing media and allowed to adhere and proliferate on the surface ...
(PBS) was then added to the wells and incubated for 1 h at 37 °C. Afterwards, culture media were added and incubated for another hour at 37 °C. HUVECs were seeded on the substrates at 1 × 104cells/cm2in serum-containing media and allowed to adhere and proliferate on the surface ...
cell culture inserts (Transwells) (Millipore) were used for separating HUVECs with MSCs or HSFs during the co-culture. HUVECs were in the lower chamber, the number ratio of MSCs or HSFs to HUVECs was 1:5. The culture medium for the co-culture is EndoGRO-VEGF Complete Culture Media. ...
dental pulp;culture;vascular endothelial growth factor A;bone regeneration;stem cells 1. Introduction Regenerative medicine has emerged as a new therapeutic alternative to transplantation that enables the regeneration of dysfunctional tissues using tissue-specific stem cells [1]. Indeed, therapies involving...