A:When choosing between the various types of HUVECs that Lonza offers, the choice can be made by answering the following questions: 答:在龙沙提供的各种类型huvec之间进行选择时,可以通过回答以下问题进行选择: What media do you intend to culture the cells in?你打算用什么培养基培养细胞 Do you requ...
The use of in vitro coculture of human umbilical vein endothelial cells (HUVECs) and human mesenchymal stem cells (hMSCs) has been one of the most explored options. Both cell types secrete specific growth factors that are mutually beneficial, and studies suggested that cell-cell communication ...
Cell culture Human umbilical vein endothelial cells (HUVECs) were maintained in endothelial basal media containing 5% fetal bovine serum and cultured in endothelial cell medium containing 1% penicillin/streptomycin solution, 1% endothelial cell growth supplement and 5% fetal bovine serum (Sciencell, USA...
We measured the BDNF level in the cultured media of various different breast cancer cell lines and the results showed that the MDA-MB-231 cells, but not the various other cell types, secrete BDNF into the culture media in a time-dependent manner (Fig. 2a). When analyzed by migration assay...
HUVECs were cultured on six-well culture plates. The cells were pretreated with resveratrol (100 μM) for 1 h and then with ox-LDL (0.1 mg/ml) for 8 h. The cells were fixed with 4% paraformaldehyde for 15 min and washed twice. The cells were then incubated with an antibo...
Cells were seeded into 96-well microtiterplates at a density of 2500 cells per well and incubated over night under standard cell culture conditions in order to allow adherence of the cells. The next day cells were treated as indicated (10 ng/mL VEGF respectively 10 ng/mL VEGF in combination...
HUVECs were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). Cells were grown on 100 × 20 mm2Petri dishes coated with 0.1% gelatin for subculture. Cultures were maintained with endothelial cell medium (Sciencell, Carlsbad, CA, USA) containing 5% fetal bovine ...
and 2 mM l-glutamine under standard culture conditions. The next day, the supernatant was discarded, the cells were washed with PBS three times, and a new medium was added. After 3–7 days, the cells crawled out of the tissue mass. The culture medium was replaced every 5 days...
The use of in vitro coculture of human umbilical vein endothelial cells (HUVECs) and human mesenchymal stem cells (hMSCs) has been one of the most explored options. Both cell types secrete specific growth factors that are mutually beneficial, and studies suggested that cell-cell communication ...
International Journal of Molecular Sciences Article Effects of Co-Culture Media on Hepatic Differentiation of hiPSC with or without HUVEC Co-Culture Nora Freyer 1,*, Selina Greuel 1, Fanny Knöspel 1, Nadja Strahl 1, Leila Amini 1, Frank Jacobs 2, Mario Monshouwer 2 and Katrin Zeilinger 1...