Lipofectamine RNAiMAX 试剂是一种专为将 Stealth™ RNAi 或短干扰 RNA (siRNA) 高效递送至哺乳动物细胞中从而进行 RNAi 分析而开发的具有专利技术的制剂。本参考文献提供了使用 Lipofectamine RNAiMAX(货号 13778-075、13778-150)将 Stealth™ RNAi 或 siRNA 转染至人脐静脉内皮...
Lipofectamine® LTX 试剂采用专有的无动物源性配方,用于将 DNA 转染到真核细胞中,且具有低细胞毒性。本参考文件提供了使用 Lipofectamine® LTX 试剂(货号 15338-100)将质粒 DNA 转染到 HUVEC 人脐静脉内皮细胞原代分离株(0 代 [Cambrex, East Rutherford,...
HUVECs were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). Cells were grown on 100 × 20 mm2Petri dishes coated with 0.1% gelatin for subculture. Cultures were maintained with endothelial cell medium (Sciencell, Carlsbad, CA, USA) containing 5% fetal bovine ...
Since BDNF in its mature form is secreted into the culture media of MDA-MB-231, this protein has the potential to affect adjacent cells associated with metastasis such as endothelial cells, thus there is a possibility of a paracrine effect. Therefore next we attempted to determine using HUVEC ...
HUVEC cells, at a density of 4 × 105, were seeded into 60 mm culture dishes. After a 24-hour treatment, cells were collected and preserved in 70 % ice-cold ethanol for 24 hours. Subsequent to this fixation, cells were washed with PBS. The cells were treated with RNase A...
Appropriate tissue culture plates and supplies .TOP Transfection of HUVEC Cells Use this procedure to transfect plasmid DNA into HUVEC cells in a 24-well format (for other formats, seeScaling Up or Down Transfections, below). All amounts an...
本参考文献提供了使用 Lipofectamine RNAiMAX(货号 13778-075、13778-150)将 Stealth™ RNAi 或 siRNA 转染至人脐静脉内皮细胞(HUVEC 细胞 - ATCC,货号 CRL-1730)中的建议程序。Lipofectamine RNAiMAX 的活性范围较宽,仅需进行少量优化工作即可实现较高敲低水平。
Protocol Recommended Reagent Amounts and Volumes Related Product Information Lipofectamine Reagents Opti-Mem Media RNAi Introduction Lipofectamine® RNAiMAX Reagent is a proprietary formulation specifically developed for highly efficient delivery of Stealth™ RNAi or s...
Protocol Recommended Reagent Amounts and Volumes Related Product Information Lipofectamine Reagents Opti-Mem Media RNAi Introduction Lipofectamine® RNAiMAX Reagent is a proprietary formulation specifically developed for highly efficient delivery of Stealth™ RNAi or sh...
Protocol Recommended Reagent Amounts and Volumes Related Product Information Lipofectamine Reagents Opti-Mem Media RNAi Introduction Lipofectamine® RNAiMAX Reagent is a proprietary formulation specifically developed for highly efficient delivery of Stealth™ RNAi or short ...