HUVEC cultures HUVECs were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). Cells were grown on 100 × 20 mm2Petri dishes coated with 0.1% gelatin for subculture. Cultures were maintained with endothelial cell medium (Sciencell, Carlsbad, CA, USA) containing 5%...
Cells were seeded into 96-well microtiterplates at a density of 2500 cells per well and incubated over night under standard cell culture conditions in order to allow adherence of the cells. The next day cells were treated as indicated (10 ng/mL VEGF respectively 10 ng/mL VEGF in combination...
2.3. Preparation and Culture of HUVECs Primary HUVECs were collected from human umbilical veins by digestion in 0.1% collagenase I in M199 medium (Gibco, NY, USA) for 15 min. The solution was collected and centrifuged at 1000 rotations per minute (rpm) for 10 min. The cells were cul...
defectiva cells (Supplementary Figure S2), suggesting that DnaK could be present on cell surfaces and also released to bacterial culture media. Figure 3. Localization of A. defectiva DnaK. The proteins cell lysate and cell envelope compartments were subjected to SDS-PAGE, and proteins were either...
by flow cytometry. The presence of TNF-α significantly decreased the expression of ZO-1. A similar tendency could be observed in the presence of AC in the culture media. A combination treatment of TNF-α and AC led to a lower expression level, compared to the control cells; however, the...
(PBS) was then added to the wells and incubated for 1 h at 37 °C. Afterwards, culture media were added and incubated for another hour at 37 °C. HUVECs were seeded on the substrates at 1 × 104cells/cm2in serum-containing media and allowed to adhere and proliferate on the surface ...
(PBS) was then added to the wells and incubated for 1 h at 37 °C. Afterwards, culture media were added and incubated for another hour at 37 °C. HUVECs were seeded on the substrates at 1 × 104cells/cm2in serum-containing media and allowed to adhere and proliferate on the surface ...
dental pulp;culture;vascular endothelial growth factor A;bone regeneration;stem cells 1. Introduction Regenerative medicine has emerged as a new therapeutic alternative to transplantation that enables the regeneration of dysfunctional tissues using tissue-specific stem cells [1]. Indeed, therapies involving...