1. Medina-Leyte, D.J., et al., Use of Human Umbilical Vein Endothelial Cells (HUVEC) as a Model to Study Cardiovascular Disease: A Review. Applied Sciences, 2020. 10(3).2. Marin V, K.G., Grès S, Farnarier C, Bongrand P, Endothelial cell culture: protocol to obtain and ...
Huvec Cell Culture We combine smartresearch with cellular tools. Contact us Intuitive We create easy-to-use products. Adaptable We build modular solutions. Durable We craft long-lasting goods. For many years our work is focused on endothelial cell physiology and pathology. Endothelial cells cultured...
1. Medina-Leyte, D.J., et al., Use of Human Umbilical Vein Endothelial Cells (HUVEC) as a Model to Study Cardiovascular Disease: A Review. Applied Sciences, 2020. 10(3). 2. Marin V, K.G., Grès S, Farnarier C, Bongrand P, Endothelial cell culture: protocol to obtain and cultiv...
1. Medina-Leyte, D.J., et al., Use of Human Umbilical Vein Endothelial Cells (HUVEC) as a Model to Study Cardiovascular Disease: A Review. Applied Sciences, 2020. 10(3). 2. Marin V, K.G., Grès S, Farnarier C, Bongrand P, Endothelial cell culture: protocol to obtain and cultiv...
To transfect HUVEC cells in different tissue culture formats, vary the amounts of Lipofectamine® LTX Reagent, DNA, cells, medium and PLUS™ Reagent used in proportion to the relative surface area, as shown in the table (amounts given ...
A.L. Cheung, Isolation and culture of human umbilical vein endothelial cells (HUVEC), Curr. Protoc. Microbiol. Append. 4 (1) (2007) (Appendix 4B).Cheung AL. Isolation and culture ofhuman umbilical vein endothelial cells (HUVEC). CUff Protoc Microbiol2007;Appendix 4, Appendix 4B....
Lipofectamine® LTX 试剂采用专有的无动物源性配方,用于将 DNA 转染到真核细胞中,且具有低细胞毒性。本参考文件提供了使用 Lipofectamine® LTX 试剂(货号 15338-100)将质粒 DNA 转染到 HUVEC 人脐静脉内皮细胞原代分离株(0 代 [Cambrex, East Rutherford,...
We recommend seeding cells recovered from cryopreservation at a density of 2.5 x 103 viable cells/cm 2.For example, three 75 cm 2 or nine 25 cm 2 tissue culture flasks can usually be established from one vial containing >5 x 105 HUVEC. The procedure given below is a sample protocol for...
SubculturingEvery 3-4 days at 8000 cells/cm2Volumes used in this protocol are for 75 cm2flasks; proportionally reduce or increase amount of dissociation solutions for culture vessels of other sizes. Remove and discard spent medium. Briefly rinse with 1X PBS (ATCC 30-2200), 1 mL/25 cm2 ...
To transfect HUVEC cells in different tissue culture formats, vary the amounts of Stealth™ RNAi or siRNA, Lipofectamine® RNAiMAX, cells, and medium used in proportion to the relative surface area, as shown below. Note: 20 µM Stealth™ RNAi or...