Optimization of Endothelial Cell (HUVEC)-Gingival Fibroblasts Co-cultures in Perfused Degradable/Polar/Hydrophobic/Ionic Polyurethane (D-PHI) ScaffoldsHUVEC-HGF co-culture in perfused D-PHI scaffolds with a 50/50 mix of media (by vol.) and greater fibroblast proportion exhibit enhanced growth, ...
Culture vessel Cells plated per well Dilution medium RNAi duplex amount Final RNAi duplex conc. Lipofectamine® RNAiMAX2 Start point Acceptable Range Reverse transf. (µl) Forward transf. (µl) Start point (pmol) Acc. Range (pmol)
To transfect HUVEC cells in different tissue culture formats, vary the amounts of Stealth™ RNAi or siRNA, Lipofectamine® RNAiMAX, cells, and medium used in proportion to the relative surface area, as shown below. Note: 20 µM Stealth™ RNAi or...
To transfect HUVEC cells in different tissue culture formats, vary the amounts of Stealth™ RNAi or siRNA, Lipofectamine® RNAiMAX, cells, and medium used in proportion to the relative surface area, as shown below. Note: 20 µM Stealth™ RNAi or...