This study represents the first step toward testing the hypothesis that hypergravity can be used as a unique resistance training modality for maintaining the health and function of skeletal muscle in microgravity. The primary objectives of this study were to use a human-powered short-arm centrifuge...
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Much has been learned about how the mammalian immune system functions at steady state and during infection using inbred mouse models. However, it has become increasingly recognized that the mouse and human immune systems differ in numerous important aspects1, thus limiting the predictive value of stu...
A 70 µm cell strainer was placed onto a new 50 ml centrifuge tube using forceps, and the resuspended cell solution was poured through the strainer to filter the cells. The filtered cell suspension was counted using a Countstar automated cell counter, and based on the cell count results, ...
Solubilized protein samples (1.4 mg total) were diluted with buffer A (see below) to have a total volume of 280 μl (5 mg/ml), sonicated (1 min) and then filtered using a Costar Spin-X 0.45 μm cellulose acetate centrifuge tube filter (Corning, Corning, NY) prior to injection (100...
Microscopic images of the molds as well as their stains situated on paper were collected using a compound microscope and commercial microscope lens for cell phone cameras, which were then used for training CNN models with a transfer learning scheme to perform the classification of mold. To enable...
(from both healthy and unhealthy donors), which are often subsequently differentiated into myotubes. For clinical applications of these cells, in vitro differentiation of muscle progenitor terminal is performed to evaluate their myogenicity. This potential is then validated with xenotransplantation and the...
A 10cm umbilical cord was taken from a sterile operating table, drained the blood and rinsed the surface blood with sterile saline. Put it into a 50 mL centrifuge tube containing 25 mL sterile PBS (Gibco, NY, USA, C2001500BT), and transfer them to a sterile operating table with a 4°...
The disease also negatively impacts on the work output of affected individuals and victims are often subjects of public ridicule [11] (Dunyo et al., 1996). The disease has been targeted for elimination by the World Health Organization (WHO) through yearly mass drug administration (MDA) of ...
Centrifuge and remove the supernatant. Resuspend the cell pellet and culture in a 24-well dish according to standard passage. Note that the entire plate must be defrosted to recover individual clones, and cryopreservation in vials is preferred. All PCR reactions (25 μL) are performed using LA...