This study represents the first step toward testing the hypothesis that hypergravity can be used as a unique resistance training modality for maintaining the health and function of skeletal muscle in microgravity. The primary objectives of this study were to use a human-powered short-arm centrifuge...
A 70 µm cell strainer was placed onto a new 50 ml centrifuge tube using forceps, and the resuspended cell solution was poured through the strainer to filter the cells. The filtered cell suspension was counted using a Countstar automated cell counter, and based on the cell count results, ...
Although PDX results are often clinically relevant, to decrease animal distress, scientists are tasked to find alternative methods that replace, reduce, and refine the use of animals [50]. Consequently, other 3D cell-based assays are widely accepted and have proven to be versatile; they cut ...
It was then carefully layered onto 25 ml of ice-cold dialyzed (against PBS) horse serum in a 50-ml plastic centrifuge tube (Falcon 2070) and the cells collected by centrifugation (700 g, 15 min, 2°C). The supernate was carefully aspirated and the pellet resuspended in 9 ml of warmed...
is readily accessible. Immune responses to many pathogens have been studied in patients, but interpreting such clinical data is difficult as numerous parameters that could influence measured immune response are often unknown. To gain better control of these critical factors, immune responses to live-at...
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Collect the single cell suspension by straining the dissociated tissue pieces through a 70 μm cell strainer. Centrifuge the cells to pellet, discard the supernatant and resuspend them in fresh DMEM medium. Enzymatic dissociation of normal lung and tumor tissue samples Timing: 90 min Alternatives:...
Beyond that, we recommend the development of space-based short-radius centrifuges and variable-gravity research facilities to investigate some questions that cannot be answered on the ground and to validate human responses to artificial gravity prescriptions in space. Whether used in the near-term to...
in the Sorvall GLC bench top centrifuge and resuspended in labeling media. For 24 hr. labeling, the cells were split into two 0.8 ml aliquots in a 24 well microtiter plate and 50 ul of the reconstituted [35 S] methionine was added to each. For 2-3 hour labeling, 5×10 cells were ...
training. Little is known about the combined effect of HR responses and AGSM effectiveness on G tolerance. A few studies have also been conducted to assess an aircrew’s tolerance under an extreme high-G load with a very high onset rate (VHOG) run in the human centrifuge for fighter ...