It has been speculated that monoclonal antibodies developed by these means could be used to formulate recombinant antivenoms that elicit fewer adverse reactions, are cost-competitive to existing therapy, and can be fine-tuned to have superior efficacy16,17,18,19,20. Phage display technology could b...
as compared with theEarth’s gravitational field for which it evolved. Investigations include the growing use ofcentrifugeslarge enough to rotatehumansubjects, as well as ingeniously automated tests of postural equilibrium for evaluating the vestibulospinal reflexes. Someastronautshave experienced relatively...
Lipids are the most abundant but poorly explored components of the human brain. Here, we present a lipidome map of the human brain comprising 75 regions, including 52 neocortical ones. The lipidome composition varies greatly among the brain regions, affe
CRITICAL: After step 11, cool the centrifuge to 4°C. Note: It is recommended that cells are stained with trypan blue before cell counting to accurately count only the viable cells. Enrichment of epithelial cells using EpCAM purification Timing: 2 h EpCAM-based enrichment is a widely used ...
The EDTA and serum samples were spun at 1,500 g for 10 minutes, using a refrigerated centrifuge (at 4°C). The plasma was pipetted into four sterile 2ml cryovials, white blood cells were aliquoted into two 2ml vials, and red blood cells were stored in two 2ml vials after being ...
After fermentation, the solution was centrifuged at 12, 000 ×g for 15 min at 4 ℃ using a refrigerated centrifuge (Jouan, France), then the supernatant was freeze-dried into a powder (LFBE) using a vacuum freeze dryer (Marin Christ, Germany). In addition, for the unfermented barley, ...
Optimized_Protocol_Human Stem Cell
Human Stem Cell Nucleofector® Kit 1 [Cat. No. VPH-5012] or the Human Stem Cell Nucleofector® Kit 2 [Cat. No. VPH-5022] if Human Stem Cell Nucleofector® Solution 2 yields the best results.Product Description Human Stem Cell Nucleofector® Kit 1 Kit 2 Cat. No. VPH-5012 VPH-...
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are challenging to obtain, unlike blood or tumour cells. As a result, cell-type signature data are often obtained from a different brain region25, species26,27, and/or a different developmental stage7than the bulk brain samples. Alternatively, cells cultured in vitro have been used19. Whether...