For tissue samples smaller than 1 cm3, use half of the volume of the enzyme mix (2.5 mL in total). Note: Ensure that the tissue pieces are in the enzymatic dissociation mix and not attached on the walls of the tube. CRITICAL: After step 11, cool the centrifuge to 4°C. Note: It ...
The suspension was centrifuged in 4 °C precooled centrifuge for 10 min at 300 × g. After centrifugation, supernatant was removed, the pellet was resuspended in 1 mL 2% FBS in HBSS, suspension was filtered using Tubes with Cell Strainer Snap Cap (Corning, 352235) and FACS (BD...
A key aspect of nearly all single-cell sequencing experiments is dissociation of intact tissues into single-cell suspensions. While many protocols have been optimized for optimal cell yield, they have often overlooked the effects that dissociation can ha
A study was made of the fire hazards peculiar to the equipment and operation of the USAF School of Aerospace Medicine Human Centrifuge and Rotational Flight simulator at Brooks Air Force Base, Texas. The study was based on present air force standards and recommendations were outlined in previous ...
The EDTA and serum samples were spun at 1,500 g for 10 minutes, using a refrigerated centrifuge (at 4°C). The plasma was pipetted into four sterile 2ml cryovials, white blood cells were aliquoted into two 2ml vials, and red blood cells were stored in two 2ml vials after being ...
After fermentation, the solution was centrifuged at 12, 000 ×g for 15 min at 4 ℃ using a refrigerated centrifuge (Jouan, France), then the supernatant was freeze-dried into a powder (LFBE) using a vacuum freeze dryer (Marin Christ, Germany). In addition, for the unfermented barley, ...
The obtained cell suspension was centrifuge, and the cell pellet plated on ultra-low attachment plates in proliferation culture media—CTS™ Neurobasal™ Medium (Gibco™) supplemented with CTS™ GlutaMAX™-I Supplement (Gibco™), CTS™ B-27™ XenoFree Supplement, 100 μg/mL ...
The final and complete formulation for Surge SFM is proprietary. A lighter version of Surge SFM containing a smaller number of additives was sometimes used (Surge SFM Light, i.e., to efficiently thaw or centrifuge keratinocytes in serum-free conditions). Fibroblasts were cultured as previously ...
Briefly, MLI samples were thawed and contents pelleted by centrifuging at 14,000g for 10 min in a bench top centrifuge. The pellets were re-suspended in 1 mL of cell lysing solution (CLS)-TC and subjected to two mechanical lysis cycles at speed 6.0 for 40 s. The extracted DNA was ...
Nucleofector® Solution for your cells using the Human Stem Cell Nucleofector® Starter Kit [Cat.No.VPH-5002]. If the Human Stem Cell Nucleofector® Solution 1 yields the best results, simply use the Human Stem Cell Nucleofector® Kit 1 [Cat. No. VPH-5012] or the Human Stem Cell ...