However, a false and often nauseating sensation of rotation, the so-called Coriolis effect, induced by the central yaw rotation, combined with the simultaneous rotation of the centrifuge cabin (passive Coriolis effect), or pilot's head (active Coriolis effect), is the main disadvantage of any ...
etc.) that mediate the degree to which zoonotic protozoan pathogens can associate with plastics. The observation that microfibers demonstrated a greater ability to associate with parasites in surrounding seawater compared to microbeads is significant because microfibers are often the most common microplas...
Sporadic Parkinson’s Disease (sPD) is a progressive neurodegenerative disorder caused by multiple genetic and environmental factors. Mitochondrial dysfunction is one contributing factor, but its role at different stages of disease progression is not fully understood. Here, we showed that neural precursor...
All biomass analyzed herein or used for further experimentation was obtained by the pilot scale production. Following harvest, a centrifugal separator was used to concentrate the microalgae suspension (Westfalia, Model SA 1-02-175) by 95 % in volume. Using a centrifuge (Sorvall, Model RC-3) wi...
Eluates were pooled and dried down using a vacuum centrifuge, then brought to 100 µL using 0.1% formic acid (FA) in 3% acetonitrile (ACN). Liquid chromatography tandem mass spectrometry Proteomic analysis of the prepared samples was performed by LC–MS/MS using an UlitMate 3000 RSLCnano ...
A mixture of the retroviral supernatant and the cell culture medium in equal amounts was added to the cell in the presence of 5 μg/ml polybrene on the plate. The cell plate was set up in the centrifuge at 35°C and then centrifuged for 30 min at 800 × g. iPSC Induction Assay ...
Note: Ensure that the tissue pieces are in the enzymatic dissociation mix and not attached on the walls of the tube. CRITICAL: After step 11, cool the centrifuge to 4°C. Note: It is recommended that cells are stained with trypan blue before cell counting to accurately count only the viab...
Samples were dried down in a vacuum centrifuge at room temperature. Samples were then reconstituted in in 2 μl 30% ACN 0.1% FA and 10 μl of 3:8 70% formic acid: 0.1% TFA as described earlier. CHK2 and threonine phosphosite library digestion Twenty-four micrograms of cleaved Thr...
are perhaps not surprising, as the venom ofD. polylepisis more complex than that ofN. kaouthia, and it is well-established that toxins other than long-chain α-neurotoxins (i.e., short-chain α-neurotoxins and dendrotoxins) play important roles in the toxicity ofD. polylepisvenom25. Where ...
After fermentation, the solution was centrifuged at 12, 000 ×g for 15 min at 4 ℃ using a refrigerated centrifuge (Jouan, France), then the supernatant was freeze-dried into a powder (LFBE) using a vacuum freeze dryer (Marin Christ, Germany). In addition, for the unfermented barley, ...