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A key aspect of nearly all single-cell sequencing experiments is dissociation of intact tissues into single-cell suspensions. While many protocols have been optimized for optimal cell yield, they have often overlooked the effects that dissociation can ha
For tissue samples smaller than 1 cm3, use half of the volume of the enzyme mix (2.5 mL in total). Note: Ensure that the tissue pieces are in the enzymatic dissociation mix and not attached on the walls of the tube. CRITICAL: After step 11, cool the centrifuge to 4°C. Note: It ...
ofcentrifugeslarge enough to rotatehumansubjects, as well as ingeniously automated tests of postural equilibrium for evaluating the vestibulospinal reflexes. Someastronautshave experienced relatively minor vestibularsymptomson returning from spaceflight. Some of these disturbances have lasted for several days,...
The cell suspension was centrifuged for 3 min at 1000 rpm in Heraeus Megafuge 16R Centrifuge (Thermo Fisher Scientific, USA), the supernatant removed and the remaining cell pellet re-suspended in epithelial medium containing 3:1 mixture of low-glucose DMEM:F12 supplemented with fetal calf serum ...
After fermentation, the solution was centrifuged at 12, 000 ×g for 15 min at 4 ℃ using a refrigerated centrifuge (Jouan, France), then the supernatant was freeze-dried into a powder (LFBE) using a vacuum freeze dryer (Marin Christ, Germany). In addition, for the unfermented barley, ...
Details of the PCR primers designed using Oligo™ 4.0 primer design package for each of the human caveolin genes are found in Table 1. The PCR amplification of caveolin-1, -2 and -3 genes was carried out as follows: initial denaturation at 95 °C for 2 min, followed by denaturation ...
The final and complete formulation for Surge SFM is proprietary. A lighter version of Surge SFM containing a smaller number of additives was sometimes used (Surge SFM Light, i.e., to efficiently thaw or centrifuge keratinocytes in serum-free conditions). Fibroblasts were cultured as previously ...
Briefly, MLI samples were thawed and contents pelleted by centrifuging at 14,000g for 10 min in a bench top centrifuge. The pellets were re-suspended in 1 mL of cell lysing solution (CLS)-TC and subjected to two mechanical lysis cycles at speed 6.0 for 40 s. The extracted DNA was ...
The potential for the use of these stems cells in the field of tissue engineering is extensive but so far few clinical applications have been designed11- 13. The reasons for the limitations in the applicability of these stem cells in the field of tissue engineering may be partly due to the...