Sporadic Parkinson’s Disease (sPD) is a progressive neurodegenerative disorder caused by multiple genetic and environmental factors. Mitochondrial dysfunction is one contributing factor, but its role at different stages of disease progression is not fully understood. Here, we showed that neural precursor...
Both full-length CHK2 and CHK2 KD tryptic digests were enriched using Thermo Scientific High-Select™ Fe-NTA Phosphopeptide Enrichment Kit (#A32992) following manufacturer’s instructions. Samples were dried in a vacuum centrifuge at room temperature and reconstituted in 2 μl 30% ACN 0.1%...
as compared with theEarth’s gravitational field for which it evolved. Investigations include the growing use ofcentrifugeslarge enough to rotatehumansubjects, as well as ingeniously automated tests of postural equilibrium for evaluating the vestibulospinal reflexes. Someastronautshave experienced relatively...
For tissue samples smaller than 1 cm3, use half of the volume of the enzyme mix (2.5 mL in total). Note: Ensure that the tissue pieces are in the enzymatic dissociation mix and not attached on the walls of the tube. CRITICAL: After step 11, cool the centrifuge to 4°C. Note: It ...
MSCs are ideal cells for injury repair. Many types of MSCs can promote tissue repair and regeneration through transplantation. However, research shows that only a small proportion of MSCs actually integrates and survives within host tissues [3,4]. Secretion of paracrine factors is a commonly accept...
For each sample, 150 μL serum was pipetted into a 2 mL centrifuge tube. Internal standard solution (30 μL, 50 ng mL− 1 for perfluorooctane sulfonic acid (PFOS) and 20 ng mL− 1 for other PFASs, Wellington Laboratories, Canada), and methanol (120 μL) were added prior to...
After fermentation, the solution was centrifuged at 12, 000 ×g for 15 min at 4 ℃ using a refrigerated centrifuge (Jouan, France), then the supernatant was freeze-dried into a powder (LFBE) using a vacuum freeze dryer (Marin Christ, Germany). In addition, for the unfermented barley, ...
ELISAThis package insert must be read in its entirety before using this product. For research use only. Not for use in diagnostic procedures.Catalog Number DC3L10For the quantitative determination of human Chitinase 3-like 1 (CHI3L1) concentrations in cell culture supernates, serum, plasma, and...
Several layers of the human brain organization potentially bridging the two research streams, however, largely escape the current scientific focus. Among these layers, we believe, is the human brain lipidome. Lipids are the main components of brain tissue, comprising 78% of the dry weight of ...
(acetonitrile supplemented with 0.1% TFA): 0% B for 5 min, 0–15% B over 10 min, 15–45% B over 60 min, 45–70% B over 10 min, and 70% B over 9 min25,31. Fractions were collected manually and evaporated using a vacuum centrifuge. Toxins were dissolved in phosphate ...