For this reason, the majority of human centrifuges are used solely as passive G-trainers in relatively short sessions. This paper discusses the development of a novel motion filter which aims to minimize the undesired Coriolis effects, by allowing for small mismatches in the alignment of pitch ...
For tissue samples smaller than 1 cm3, use half of the volume of the enzyme mix (2.5 mL in total). Note: Ensure that the tissue pieces are in the enzymatic dissociation mix and not attached on the walls of the tube. CRITICAL: After step 11, cool the centrifuge to 4°C. Note: It ...
Lipids are the most abundant but poorly explored components of the human brain. Here, we present a lipidome map of the human brain comprising 75 regions, including 52 neocortical ones. The lipidome composition varies greatly among the brain regions, affe
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ofcentrifugeslarge enough to rotatehumansubjects, as well as ingeniously automated tests of postural equilibrium for evaluating the vestibulospinal reflexes. Someastronautshave experienced relatively minor vestibularsymptomson returning from spaceflight. Some of these disturbances have lasted for several days,...
A mixture of the retroviral supernatant and the cell culture medium in equal amounts was added to the cell in the presence of 5 μg/ml polybrene on the plate. The cell plate was set up in the centrifuge at 35°C and then centrifuged for 30 min at 800 × g. iPSC Induction Assay ...
After fermentation, the solution was centrifuged at 12, 000 ×g for 15 min at 4 ℃ using a refrigerated centrifuge (Jouan, France), then the supernatant was freeze-dried into a powder (LFBE) using a vacuum freeze dryer (Marin Christ, Germany). In addition, for the unfermented barley, ...
Optimized_Protocol_Human Stem Cell
an in vitro methodology that can be used to actively select for antibodies with high-affinity and cross-reactivity14,15. In addition, the use of human antibody libraries in combination with phage display technology allows for the discovery of fully human antibodies that are likely to have high ...
Mice engrafted with components of a human immune system have become widely-used models for studying aspects of human immunity and disease. However, a defined methodology to objectively measure and compare the quality of the human immune response in diffe