The center of the enlarged cell constricts until two daughter cells are formed, each offspring receiving a complete copy of the parental genome and a division of the cytoplasm (cytokinesis). This process of cytokinesis and cell division is directed by a protein called FtsZ. FtsZ assembles into ...
Thioredoxin (Trx), maltose-binding protein (MBP), glutathione S-transferase (GST) and ubiquitin (Ub) are among the most widely used solubility tags that accumulate to high levels in the E. coli cytoplasm and have proven to markedly increase the solubility of many heterologous proteins13,14,15...