3A). Additionally, short hairpin RNAs (shRNAs) can be expressed in a lentiviral context to accomplish stable RNAi in cells [154]. RNAi is most active in the cytoplasm, which makes it most useful for targeting mRNAs and lncRNAs that reside in the cytoplasm [155]. Success in knocking down ...
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In order to determine the EE of liposomes, a first step is to separate the unencapsulated drug fraction from the encapsulated drug. This can be achieved using size-exclusion chromatography [54]. This method separates the two fractions based on the differences in the size between the drug-...