Axelsson, H. 1999. Cell separation, centrifugation. I n Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis and Bioseparation. M.C. Flickinger and S.W. Drew, editors. John Wiley and Sons. 513-531.Axelsson, H.: Cell separation, centrifugation. In: Flickinger, M.; Drew, C.: ...
Axelsson H. 1999. Cell separation, centrifugation. In: Flickinger MC, Drew SW, editors. Encyclopedia of bioprocess technology--Fermentation, biocatalysis, and bioseparation. New York: Wiley & Sons. p 513-531.Axelsson, H.: Cell separation, centrifugation. In: Flickinger, M.; Drew, C.: ...
These cells were isolated from rat liver by a simple and rapid procedure involving: (1) cell separation by centrifugation on a Nycodenz gradient, after dispersion of the liver cells by collagenase perfusion; (2) further purification of... P Heldin,H?kan Pertoft,H Nordlinder,... - 《Experi...
Buffy coat preparations containing peripheral blood mononuclear cells (PBMCs) were diluted 1:1 in preparation buffer (PBS with 0.1% BSA and 2 mM EDTA), and mononuclear cell separation was performed by density centrifugation (Ficoll-Paque Premium 1.084, Cytiva) with diluted peripheral blood cells...
(Fig.7f) gene signatures were the most significant contributors to cluster separation in the TCGA cohort, while NEPC-downregulated genes were the major determinant in the ICGC cohort (Supplementary Fig.7c). In line with previous reports2, signatures reflecting AR activity (AR activity and full ...
Fresh peripheral blood was collected prior to surgery, and then peripheral blood mononuclear cells (PBMCs) were isolated using lymphocyte separation medium (Human) (Solarbio). The dissociated cells were filtered through a 100 μm cell strainer in DPBS and collected by centrifugation at 300 ×...
Cell separation and culture The isolation of ESCs was carried out from in vitro culture of blastodermal cells taken from the area pellucida of Stage X (EG&K) embryos. The isolated blastoderm cells were cultured in DMEM supplemented with 10% FBS, 1000 IU/ml LIF, 10 ng/ml bFGF, 10 ng/...
For nuclear-cytoplasmic separation, cells were collected and fractionated using a Nuclear/Cytosol Fractionation Kit (FUDE, FD0199, Hangzhou, China) according to the manufacturer's protocol. Briefly, cells were trypsinized, washed with PBS, and resuspended in cytoplasmic extraction buffer A. After inc...
(Fig.1e). Skin layers and cells segregated by the first and second component with 24.0% and 11.0% of the total data variation, respectively. The separation between skin layers and skin-derived immune cells was partially driven by proteins of the ECM, including collagens and collagen associated...
Dotted line indicates the separation between cortex (c) and medulla (m). A higher magnification showing that KRT15 is expressed at low levels in KRT8+KRT5+ immature TECs and at higher level in mTECs is shown in the right panels. Medullary area is marked with “m” while cortical area is...