Assessment of the impact of variation in chloroplast and mitochondrial DNA (collectively termed the plasmotype) on plant phenotypes is challenging due to the difficulty in separating their effect from nuclear-derived variation (the nucleotype). Haploid-inducer lines can be used as efficient plasmotype...
A way had been found to recognize infectious blood donations and to sort them out. Blumberg used the agar gel double diffusion developed by Ouchterlony in the discovery of AuAg. Compared to the biochemical methods of protein determination, the Ouchterlony method was fairly sensitive and specific...
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Using targeted infection, New York pediatrician Saul Krugman showed 1967 that there were indeed two separate pathogens causing hepatitis [6]. However, even this experimentation - unimaginable by today’s ethical standards - did not lead to the urgently needed break-through. Discovery of Australia ...
Thioredoxin (Trx), maltose-binding protein (MBP), glutathione S-transferase (GST) and ubiquitin (Ub) are among the most widely used solubility tags that accumulate to high levels in the E. coli cytoplasm and have proven to markedly increase the solubility of many heterologous proteins13,14,15...
The histories of research regarding all plant hormones are similar. Identification and structural elucidation have been followed by analyses of their biosy
Connective tissue growth factor (CTGF/CCN2) is a member of the CCN family of matricellular proteins. Its expression is induced by a number of factors inclu
In order to determine the EE of liposomes, a first step is to separate the unencapsulated drug fraction from the encapsulated drug. This can be achieved using size-exclusion chromatography [54]. This method separates the two fractions based on the differences in the size between the drug-...