Microbe-seq : high-throughput, single-microbe genomics with strain resolution, applied to a human gut microbiome 来自 钛学术 喜欢 0 阅读量: 375 作者:W Zheng,S Zhao,Y Yin,H Zhang,DA Weitz 摘要: We present Microbe-seq , a high-throughput single-microbe method that yields strain-resolved ...
High-throughput, single-microbe genomics with strain resolution, applied to a human gut microbiome. Science 376, eabm1483 (2022). Article CAS PubMed Google Scholar Lloréns-Rico, V., Simcock, J. A., Huys, G. R. B. & Raes, J. Single-cell approaches in human microbiome research. ...
7,8,9,10. The key strategy of current prevalent droplet-based scRNA-seq systems, such as 10X Genomics Chromium platform7, is using droplet microfluidics to co-encapsulate a single cell with a uniquely DNA barcoded bead in a droplet,
哈佛大学和麻省理工学院的研究团队在微生物群落研究方法学上取得重要突破,发明了微生物高通量单细胞基因组学技术—Microbe-seq。相关成果以研究长文(Research Article)的形式在Science上以High-throughput, single-microbe genomics with strain resolution, applied to a human gut microbio ...
SOAP http://soap.genomics.org.cn/ SWIFT http://bibiserv.techfak.uni-bielefeld.de/swift Vmatch http://www.vmatch.de/ ZOOM http://www.bioinfor.com/zoom/general/overview.html De Novo assembly AbySS http://www.bcgsc.ca/platform/bioinfo/software/abyss ALLPATHS-LG ftp://ftp.broadinstitute.or...
Processing of 10x Genomics single-cell BCR reads We removed sequences with nonproductive arrangements and filtered for cells with exactly one heavy chain sequence. B cell clones were inferred based on heavy chain sequences using hierarchical clustering with single-linkage [26] for each subject. Speci...
Genomics. 2018;110(5):231–8. Article CAS PubMed Google Scholar Fernández-Gómez B, Richter M, Schüler M, Pinhassi J, Acinas SG, González JM, Pedrós-Alió C. Ecology of marine Bacteroidetes: a comparative genomics approach. The ISME Journal. 2013;7(5):1026–37. Article CAS Pub...
Data availability The raw sequencing data and original analytic code have been deposited in the OMIX, China National Center for Bioinformation/Beijing Institute of Genomics, Chinese Academy of Sciences (https://ngdc.cncb.ac.cn/omix: accession No. OMIX002658)....
The genomic revolution has provided the DNA code as a condensed set of cell instructions that constitutes the main engineering target, and functional genomics to understand the cell behavior. Furthermore, the cost for these data is rapidly decreasing: sequencing cost decreases faster than Moore's la...