We present Microbe-seq , a high-throughput single-microbe method that yields strain-resolved genomes from complex microbial communities. We encapsulate individual microbes into droplets with microfluidics and liberate their DNA, which we amplify, tag with droplet-specific barcodes, and sequence. We use...
High-throughput, single-microbe genomics with strain resolution, applied to a human gut microbiome. Science 376, eabm1483 (2022). Article CAS PubMed Google Scholar Lloréns-Rico, V., Simcock, J. A., Huys, G. R. B. & Raes, J. Single-cell approaches in human microbiome research. ...
Microfluidic has been widely used for high-throughput single-cell analysis18,19. Our previous work has established a microfluidic droplet barcoding platform and developed inDrop20, a high-throughput method for single-cell RNA sequencing, and Microbe-seq21, a high-throughput method for single-microbe...
哈佛大学和麻省理工学院的研究团队在微生物群落研究方法学上取得重要突破,发明了微生物高通量单细胞基因组学技术—Microbe-seq。相关成果以研究长文(Research Article)的形式在Science上以High-throughput, single-microbe genomics with strain resolution, applied to a human gut microbio ...
Processing of 10x Genomics single-cell BCR reads We removed sequences with nonproductive arrangements and filtered for cells with exactly one heavy chain sequence. B cell clones were inferred based on heavy chain sequences using hierarchical clustering with single-linkage [26] for each subject. Speci...
To the best of our knowledge, this is the first high-throughput amplicon sequencing study on the microbial community structure and diversity in C. lentillifera. The findings from such investigations may shed light on the cause and process of disease outbreaks in C. lentillifera and such knowledge...
Computational Biology and Genomics Tools for Biohydrogen Research Meganathan P. Ramakodi, in Biohydrogen (Second Edition), 2019 4 Conclusion and Perspectives The field of high-throughput sequencing technology is continuously evolving and the cost of DNA/RNA sequencing is coming down gradually. In addit...
Automated Sample Preparation for High-Throughput Single-Cell Proteomics (2018) Google Scholar 14. Potriquet J., Laohaviroj M., Bethony J. M., Mulvenna J. A modified FASP protocol for high-throughput preparation of protein samples for mass spectrometry PLoS One, 12 (2017), p. 10.1371/journ...
Drop-BS takes advantage of the ultrahigh throughput offered by droplet microfluidics to prepare bisulfite sequencing libraries of up to 10,000 single cells within 2 days. We apply the technology to profile mixed cell lines, mouse and human brain tissues to reveal cell type heterogeneity. Drop-...
The CAMII platform consists of four key elements (Fig.1a) discussed as follows: (1) an imaging system that collects morphology data of colonies and an AI-guided colony selection algorithm, (2) an automated colony-picking robot for high-throughput isolation and arraying of isolates, (3) a co...