Shalem O, Sanjana NE, Zhang F. High-throughput functional genomics using CRISPR- Cas9. Nat. Rev. Genet. 2015;16(5):299-311.Shalem, O., Sanjana, N.E. and Zhang, F. (2015) High-throughput functional genomics using CRISPR-Cas9. Nat Rev Genet, 16, 299-311....
In this study, high-throughput functional genomics screens were used to identify and validate synthetic lethal combinations in a panel of colorectal cancer cell lines. An overview of the experimental design is shown inFig. 1. The screen contained four experimental arms: the DMSO anchor arm, to a...
prime editing sensor screens could be used to systematically identify variant-specific vulnerabilities to diverse therapies, augmenting cDNA-based approaches for performing similar screens54. Prime editing reveals new pathogenic variants High-throughput functional genomics approaches have been used previously to...
High-throughput functional genomic screens identify key transcription factors from inferred GRNs that are functionally relevant for cardiomyocyte lineages derived from each pathway. Notably, we discover a critical heat shock transcription factor 1 (HSF1)-mediated cardiometabolic GRN controlling cardiac ...
High-throughput functional genomics using CRISPR–Cas9. Nat. Rev. Genet. 16, 299–311 (2015). Google Scholar Kuhn, M., Santinha, A. J. & Platt, R. J. Moving from in vitro to in vivo CRISPR screens. Gene Genome Editing 2, 100008 (2021). Google Scholar Fernandes Neto, J. M. ...
Gene-level meta-analysis of high-throughput functional genomics (RNAi or CRISPR) screens. Resources Readme License MIT license Activity Stars 9 stars Watchers 2 watching Forks 8 forks Report repository Releases No releases published Packages No packages published Languages R 100.0% Foote...
Here, we enable high-throughput pooled screens of thousands of DNA variants in the human genome by lentiviral delivery of PE, namely PRIME. We demonstrate the utility of PRIME for three different applications, including the single-base resolution analysis of a 716 bp enhancer, the functional char...
High-throughput functional genomics using CRISPR–Cas9 Forward genetic screens are powerful tools for the discovery and functional annotation of genetic elements. Recently, the RNA-guided CRISPR (clustered regularly interspaced short palindromic repeat)-associated Cas9 nuclease has been comb... O Shalem,...
In two papers published today in Cell, two teams working collaboratively from UCSF and the Broad Institute describe Perturb-seq, a novel approach to high-throughput functional genomics enabled by the combination of single cell RNA-seq with CRISPR perturbations. Both teams took advantage of the mass...
High-throughput characterization of HLA-E-presented CD94/NKG2x ligands reveals peptides which modulate NK cell activation Brooke D. Huisman Ning Guan Michael E. Birnbaum Nature Communications (2023) The genomics revolution comes to the immunopeptidome Peter M. Bruno Genes & Immunity (2023) ...