7,8,9,10. The key strategy of current prevalent droplet-based scRNA-seq systems, such as 10X Genomics Chromium platform7, is using droplet microfluidics to co-encapsulate a single cell with a uniquely DNA barcoded bead in a droplet,
single cell genomicsunculturable bacteriamicrobiomemicrobial ecologyprimary template-directed amplificationmultiple displacement amplificationwhole genome amplificationmicrobiome sequencingmobile genetic elementsbiosynthetic gene clustersMicrobial genomes produced by standard single-cell amplification methods are largely ...
Basic genetic information such as GC content, number of ORFs, and prediction of ORF function was analysed by CLgenomics software (http://www.chunlab.com). The CDSs were classified into different groups based on their roles, with reference to orthologous groups (EggNOG 4.1; http://eggnogdb....
and it can also be scaled up for use with multiple samples and applicable to a variety of samples with the presence of microbes. We validated this protocol by successfully generating a barcoded library using the 10× Genomics microfluidic platform from tissue subjected to this procedure. This wor...
哈佛大学和麻省理工学院的研究团队在微生物群落研究方法学上取得重要突破,发明了微生物高通量单细胞基因组学技术—Microbe-seq。相关成果以研究长文(Research Article)的形式在Science上以High-throughput, single-microbe genomics with strain resolution, applied to a human gut microbio ...
Therefore, isolation of microbe-free, high-quality nuclei from plant tissues are necessary to avoid clogging or interference with the microfluidic channel (e.g., 10脳 Genomics) or particle-templated emulsion that are used in sNucRNA-seq platforms. Here, we developed a simple...
Single-cell genomics reveals complex carbohydrate degradation patterns in poribacterial symbionts of marine sponges. ISME J. 2013;7:2287–300. Article CAS PubMed PubMed Central Google Scholar Horn H, Slaby BM, Jahn MT, Bayer K, Moitinho-Silva L, Förster F, et al. An enrichment of ...