cell–cell communications, spatial single-cell omics, barcode lineage tracing, single-cell multi-omics, multiple perturbations with single-cell readout, immunoprofiling, and clinical application (Fig.1). In this review, I discuss the recent advances and future perspectives of single-cell genomics in...
Single-cell genomics meets human genetics In this Review, the authors describe the emerging field of single-cell genetics, which lies at the intersection of single-cell genomics and human genetics. They review the first single-cell expression quantitative trait loci studies, which combine single-cell...
Cellular heterogeneity is revolutionizing the way to study, monitor and dissect complex diseases. This has been possible with the technological and computational advances associated to single-cell genomics and epigenomics. Deeper understanding of cell-to-cell variation and its impact on tissue function wi...
To study such changes directly in the germline of mammals requires access to material as well as single cell genomics. Recent improvements in embryology and single-cell DNA amplification make it possible to study the genomic changes directly in human oocytes, sperm, and preimplantation embryos. This...
Single-Cell Genomics With over 40 trillion cells in the human body and hundreds of cell types, cells have nevertheless traditionally been studied using bulk methods at the cell population or tissue level rather than single-cell level. However, the field of genomics and computational biology has ...
Space Ranger (version 1.1.0, 10x Genomics) was used for the read-mapping to the human reference genome (GRCh38) with default parameters. Anatomical microstructures were manually annotated using the paired histology H&E image. To map clusters onto Visium results, we used Cell2Location67. In ...
of single cell genomics at the Center for Epigenomics at University of California, San Diego. As a postdoctoral fellow in Dr. Bing Ren's laboratory at the Ludwig Institute for Cancer Research he studied brain development and cancer using single cell ATAC-seq and single cell RNA-seq approaches....
The LCM technique has been extensively used in combination with genomics, transcriptomics and proteomics analyses [18, 19], but only very rarely for multi-element analysis [20]. Samples can be either from heterogeneous mixtures of cell populations or from dry sections mounted on culture plates or...
The ability of single-cell sequencing to delineate the genomics and transcriptomics of circulating and disseminated cancer cells holds great promise for making meaningful improvements in personalized oncology over the next several years. To date, SCS has been used primarily in the research setting; ...
Genome Biology (2016) 17:83 DOI 10.1186/s13059-016-0939-7 RESEARCH Open Access Single-cell profiling of human megakaryocyte-erythroid progenitors identifies distinct megakaryocyte and erythroid differentiation pathways Bethan Psaila1,2,3, Nikolaos Barkas3†, Deena Iskander1†, Anindita Roy3,4, ...