Temple, Texas, USAObjective: To describe leptomeningeal myelomatosis (LM) in previously treated high-risk kappa light chain multiple myeloma (MM) and to review the literature.Case report: A 71-year-old female with previously treated kappa light chain myeloma presented with right lumbosacral discomfo...
The serum free light chain (FLC) assay quantitates free kappa (κ) and free lambda (λ) immunoglobulin light chains. This assay has prognostic value in pla... MJ Maurer,INM Micallef,JR Cerhan,... - 《Journal of Clinical Oncology》 被引量: 93发表: 2011年 N Latex FLC serum free light...
For example, conversion of Phe90 in the murine kappa light chain variable domain germline sequence IgkV9-124*01 to TyrL91 in 10G4 provided a hydrogen bond donor that stabilized a water-mediated contact to the C5-OH of the LTC4 arachidonic acid moiety, while mutation in the IgHV3-1*02 ...
We identified changes at 13.6% of the amino acid positions in the V gene of the antibody heavy chain, at 8.4 % in the kappa and at 10.6 % in the lambda V gene. We identified somatic insertions or deletions in 8.1% of the variable genes. We also found several small groups of clonal ...
In a preferred embodiment, antigen is administered directly to mice which comprise DNA loci encoding both human Ig heavy chain variable region and kappa light chain variable region (Veloclmmune®), with an adjuvant to stimulate the immune response. Such an adjuvant includes complete and incomplete...
We characterized the heavy chain and kappa light chain antibody repertoires of a model animal, the OmniRat, by high throughput antibody sequencing and made use of two novel datasets for comparison to human repertoires. Intra-animal and inter-animal repertoire comparisons reveal a high level of ...
An example causal assertion is the increased transcriptional activity of NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) causes an increase in the mRNA expression of CXCL1 (Chemokine (C-X-C motif) ligand 1) [HeLa cell line; Human; PMID 16414985]. The knowledgebase ...
A single cell-based 5′ RACE PCR of these R4-gated cells resulted in the amplification of cognate pairs of VH and light chain kappa variable (VL) genes. DNA transfection of pairs of immunoglobulin heavy (IgH) and immunoglobulin light (IgL) genes into 293 cells resulted in the production of...
The murine kappa light chain Upstream of both of these regions are unique multiple cloning sites (MCS) that enable the insertion of the variable regions of any given mAb. Back to the top Specifications Heavy chain cassette: Composite promoter:Aldehyde dehydrogenase (AldA) enhancer/ Human ferritin ...
A single cell-based 5′ RACE PCR of these R4-gated cells resulted in the amplification of cognate pairs of VH and light chain kappa variable (VL) genes. DNA transfection of pairs of immunoglobulin heavy (IgH) and immunoglobulin light (IgL) genes into 293 cells resulted in the production of...